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Bacillus polymyxa special fertilizer and preparation method and application thereof

A special technology of Bacillus polymyxa, applied in the field of biological fertilizer, can solve the problems of increasing the cost of pest control, reducing the commercial value of fruit, harming citrus leaves, etc., so as to improve the quality of agricultural products, prevent and treat citrus canker, and improve the quality and yield. Effect

Active Publication Date: 2020-06-19
广西乐土生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus; the disease damages citrus leaves, branches and fruits, and when seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, affecting tree vigor; The fruit will drop, and the light one will have disease scars, which will not tolerate storage and rot, which will greatly reduce the commodity value of the fruit, increase the cost of disease and pest control for the fruit grower, and damage the economic benefits.
[0003] At present, P. flavus is usually controlled by spraying pesticides on citrus leaves. However, on the one hand, pesticides can easily make pathogenic bacteria resistant and reduce the difficulty of control; on the other hand, they will pollute the environment.

Method used

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  • Bacillus polymyxa special fertilizer and preparation method and application thereof
  • Bacillus polymyxa special fertilizer and preparation method and application thereof
  • Bacillus polymyxa special fertilizer and preparation method and application thereof

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Effect test

Embodiment 1

[0025] The screening of embodiment 1 bacillus polymyxa LTPP03

[0026] 1) Sample collection: collect soil (forest land in Sitang Town, Xingning District, Nanning City), and place it in a container;

[0027] 2) Dilution, water bath heating: Take 10g of soil and put it into a 250ml beaker, add 90ml of sterile water into the beaker, shake it, and make the concentration of 10 -1 Soil dilution A; place soil dilution A in a water bath at 80°C and heat for 10 minutes;

[0028] 3) Gradient dilution:

[0029] (1) Take 3 test tubes filled with 9mL sterile water, marked as: 10 -2 、10 -3 and 10 -4 ;

[0030] (2) Shake the soil diluent A and let it rest for 30s. Use a pipette to draw 1mL of the soil diluent and add it to the water marked with 10 -2 In the test tube, gently blow and suck several times in the test tube to make it fully mixed, and then obtain the soil dilution B;

[0031] (3) Similarly, draw 1mL of soil dilution B and add the number 10 -3 In a test tube, blow and suck...

Embodiment 2

[0043] The identification of embodiment 2 bacillus polymyxa LTPP03

[0044] The China Center for Type Culture Collection identified the Bacillus polymyxa LTPP03 strain on March 15, 2019. Identification includes morphological observation of Bacillus polymyxa LTPP03 strain, physiological and biochemical characteristics test, determination and analysis of 16SrRNA gene sequence, and the taxonomic status of the strain is determined according to the identification results. The strain was identified as: Paenibacillus polymyxa LTPP03 (Paenibacillus polymyxa LTPP03). The test results of physiological and biochemical characteristics are shown in Table 1-Table 2;

[0045] Table 1 Physiological and biochemical characteristics of strain LTPP03-enzyme activity, carbon source assimilation

[0046]

[0047]

[0048] +: Positive reaction; -: Negative reaction; w: Weak positive reaction

[0049] Table 2 Physiological and biochemical characteristics of strain LTPP03—acid production using ...

Embodiment 3

[0054] A preparation method of bacillus polymyxa special fertilizer, comprising the steps of:

[0055] 1) Weighing: Weigh 5 billion spores / gLTPP03 bacterial powder 30g, 100 billion spores / g Bacillus amyloliquefaciens bacterial powder 1g, diammonium phosphate 100g, nitrogen and potassium fertilizer 150g, potassium sulfate 100g, potassium dihydrogen phosphate 50g, monohydrate Magnesium sulfate 224g, borax pentahydrate 25g, sodium methylene bis-naphthalene sulfonate 20g, sodium lignosulfonate 40g, humic acid raw powder 240g, polyacrylamide 10g and fatty alcohol polyoxyethylene ether 10g;

[0056] 2) Mixing: mixing magnesium sulfate monohydrate, borax pentahydrate, Bacillus amyloliquefaciens bacteria powder, LTPP03 bacteria powder, sodium methylene bis-naphthalene sulfonate and sodium lignosulfonate in a blender to obtain a mixture;

[0057] 3) Mixing: Stir ammonium sulfate, nitrogen and potassium fertilizer, potassium sulfate, potassium dihydrogen phosphate and diammonium phospha...

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Abstract

The invention discloses a bacillus polymyxa special fertilizer and a preparation method and application thereof. The special fertilizer comprises bacillus polymyxa powder, the bacillus polymyxa is preserved in China Center for Type Culture Collection, the preservation number is CCTCC No: M2019116, the bacillus polymyxa is named as LTPP03, and the 16srRNA sequence is shown as SEQ ID No. 1. The preparation process comprises the steps of weighing, mixing and packaging, and the special fertilizer can effectively prevent and treat citrus canker, reduce the morbidity of the citrus canker and effectively promote the growth of plants.

Description

technical field [0001] The invention relates to the technical field of biological fertilizers, and more specifically relates to a Bacillus polymyxa special fertilizer and its preparation method and application. Background technique [0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus; the disease damages citrus leaves, branches and fruits, and when seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, affecting tree vigor; The fruit falls, and the light one has disease scars and cannot be stored, and rots, which greatly reduces the commodity value of the fruit, increases the cost of disease and insect pest control for the fruit grower, and damages the economic benefits. [0003] At this stage, P. flavus is usually controlled by spraying pesticides on citrus leaves. However, on the one hand, pesticides are likely to make pathogenic bacteria resistant and reduce the difficulty of control; on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/30C05G3/80
CPCC05B7/00C05G3/00
Inventor 吕昆明廖华廖博杨瑞青周伟东陆可敬邓多桓
Owner 广西乐土生物技术有限公司
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