Dressing with functions of hemostasis and accelerated wound healing and preparation method thereof

A wound healing and functional technology, applied in DNA preparation, pharmaceutical formulations, bandages, etc., can solve the problems of complex drug formulations, slow wound healing, and expensive components, and achieve ideal safety, stable curative effect, and simple process effects.

Active Publication Date: 2022-04-22
恒昱生物制药(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although they have a certain hemostatic effect, their limitations are also very prominent: they only have the effect of one or two stages in the wound healing process, and cannot take care of multiple stages at the same time and work together; the drug formula is complex and has many components; Some drug components contain toxic ingredients; slow hemostasis and slow wound healing; inconvenient to use and carry; special and expensive components

Method used

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  • Dressing with functions of hemostasis and accelerated wound healing and preparation method thereof
  • Dressing with functions of hemostasis and accelerated wound healing and preparation method thereof
  • Dressing with functions of hemostasis and accelerated wound healing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A band-aid includes a drug carrier and a drug solution, the drug solution contains small molecule polydeoxyribonucleotides and borneol.

[0069] The preparation method of described band-aid comprises the steps:

[0070] 1. The salmon small molecule polydeoxyribonucleotide (SMPDRN) used in the hemostatic dressing is prepared, comprising the following steps:

[0071] (1) Accurately weigh 100g of salmon testis, add 600ml of alkaline lysate (200mmol NaCl, 2mol / L NaOH, 20mg / ml protein K), quickly break up the tissue, mix it upside down, and bathe in constant temperature water at 95°C for 20min.

[0072] (2) Ice bath to below 25°C, add 2 mol / L Tris-HCl (pH 8.0), the added volume accounts for 1 / 2 of the volume of the reaction system obtained in step (1), and mix well by inversion.

[0073] (3) Add 1 mol / L HCL, the added volume accounts for 1 / 3 of the volume of the reaction system obtained in step (2), and mix evenly by inverting.

[0074] (4) Centrifuge at 6000rpm for 15min ...

Embodiment 2

[0084] A band-aid, comprising a drug carrier and a drug solution, the drug solution contains small molecule polydeoxyribonucleotides and mupidocin.

[0085] The preparation method of described band-aid comprises the steps:

[0086] 1. The salmon small molecule polydeoxyribonucleotide (SMPDRN) used in the hemostatic dressing is prepared, comprising the following steps:

[0087] (1) Accurately weigh 100g of salmon semen, add 600ml of alkaline lysate (200mmol NaCl, 2mol / L NaOH, 20mg / ml protein K), quickly break up the tissue, mix it upside down, and bathe in a constant temperature water bath at 95°C for 40min.

[0088] (2) Ice bath to below 25°C, add 2 mol / L Tris-HCl (pH 8.0), the added volume accounts for 1 / 2 of the volume of the reaction system obtained in step (1), and mix well by inversion.

[0089] (3) Add 1 mol / L HCL, the added volume accounts for 1 / 3 of the volume of the reaction system obtained in step (2), and mix evenly by inverting.

[0090] (4) Centrifuge at 6000rpm...

Embodiment 3

[0100] A band-aid, comprising a drug carrier and a drug solution, the drug solution contains small molecule polydeoxyribonucleotides and mupidocin.

[0101] The preparation method of described band-aid comprises the steps:

[0102] 1. The salmon small molecule polydeoxyribonucleotide (SMPDRN) used in the hemostatic dressing is prepared, comprising the following steps:

[0103] (1) Accurately weigh 100 g of salmon ovary tissue, add 800 ml of alkaline lysate (50 mmol NaCl, 1 mol / L NaOH, 5 mg / ml protein K), quickly break up the tissue, mix by inverting, and place in a constant temperature water bath at 90°C for 40 minutes.

[0104] (2) Ice bath to below 25°C, add 1 mol / L Tris-HCl (pH 8.0), the added volume accounts for 1 / 2 of the volume of the reaction system obtained in step (1), and mix well by inversion.

[0105] (3) Add 4 mol / L HCL, the added volume accounts for 1 / 5 of the volume of the reaction system obtained in step (2), and mix evenly by inversion.

[0106] (4) Centrifuge...

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Abstract

The invention discloses a dressing with functions of stopping bleeding and accelerating wound healing, which comprises small molecule polydeoxyribonucleotide (SMPDRN) extracted from salmon. The SMPDRN is extracted from salmon tissues, and the salmon tissues include salmon testis, semen, and eggs. Experiments of the present invention have proved that the effects of hemostasis, pain relief, anti-inflammation, promotion of wound healing and the like can be achieved. The small molecule polydeoxyribonucleotide (SMPDRN) used in the present invention is obtained by the preparation method provided in the present invention, and has the characteristics of molecular weight concentrated in the high-efficiency region, relatively simple components, high purity, simple process, and stable components. It meets the needs of the preparation of hemostatic dressings, enables the hemostatic dressings to obtain stable curative effect and ideal safety, overcomes the difficulty of animal preparations in the preparation of hemostatic dressings, and develops small molecule polydeoxyribonucleotides in the field of medicine of new applications.

Description

technical field [0001] The invention relates to a dressing with functions of hemostasis and accelerated wound healing and a preparation method thereof. Background technique [0002] Traumatic blood loss can lead to pain, coma, and shock in patients. At the same time, trauma can cause local inflammation, cause delayed healing, and form abnormal scars and keloids; severe infections can cause toxemia and sepsis, which seriously endanger human health and life safety. According to statistics, massive blood loss is the main cause of 50% of war casualties. In addition, excessive blood loss is also a key factor in the increase in accident casualties in peacetime. Quickly stop bleeding, accelerate collagen synthesis, re-epithelialize, and seal the epidermis. On the one hand, it can reduce blood loss and win treatment time; on the other hand, it can maintain a stable internal environment, reduce infection, ensure normal healing, and obtain a good prognosis. Effect. [0003] At pres...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10C12N15/11A61L15/20A61L15/42A61L15/44
CPCA61L15/20A61L15/44A61L15/42A61L2400/04A61L2300/258A61L2300/402A61L2300/404
Inventor 王超云高原
Owner 恒昱生物制药(山东)有限公司
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