Bifidobacterium longum BL986, freeze-dried powder prepared from same and application of freeze-dried powder

A technology of Bifidobacterium longum and BL986, applied in the direction of bifidobacteria, bacteria used in food preparation, application, etc., can solve the problems of uneven distribution, difficult dissolution, etc., to reduce the difficulty of dispersion, good dispersion effect, and protect the activity. Effect

Inactive Publication Date: 2020-05-22
SYNBIOTECH BIOTECHNOLOGY YANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems that the existing lactic acid bacteria freeze-dried powder is difficult to dissolve and unevenly distributed in the production process of lactic acid bacteria chocolate, the present invention provides a kind of Bifidobacterium longum BL986, the freeze-dried powder prepared therefrom and the composition of the freeze-dried powder. application

Method used

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  • Bifidobacterium longum BL986, freeze-dried powder prepared from same and application of freeze-dried powder
  • Bifidobacterium longum BL986, freeze-dried powder prepared from same and application of freeze-dried powder

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Preparation of medium: Dissolve 15g of peptone, 8g of yeast extract, 15g of glucose and 0.5mL of Tween 80 in 1000mL of distilled water, and sterilize at 125°C for 15 minutes;

[0040] (2) Activation of lactic acid bacteria: insert Bifidobacterium longum BL986 into the sterilized medium, so that the number of original bacteria is 1.0×10 5 cfu / ml; ferment at 44°C for 72 hours to make the number of bacteria >5.0×10 9 cfu / ml;

[0041] (3) Cell collection: collect the lactic acid bacteria fermentation liquid, centrifuge at 7500r / min, 4°C for 12 minutes, discard the supernatant to collect the cell sediment;

[0042] (4) Freeze-drying of bacteria slime: Add freeze-drying protective agent and water to the precipitate collected in step (3), 90% of bacteria slime, 2% of skim milk powder, 8% of water, spread the thickness of 3cm, at -85°C Freezing overnight, vacuum freeze-drying with a vacuum freeze dryer for 30 hours to obtain a freeze-dried powder semi-finished product, th...

Embodiment 2

[0045] (1) Preparation of medium: Dissolve 8 g of peptone, 3 g of yeast extract, 15 g of glucose and 2 mL of Tween 80 in 1000 mL of distilled water, and sterilize at 120°C for 15 minutes;

[0046] (2) Activation of lactic acid bacteria: insert Bifidobacterium longum BL986 into the sterilized medium, so that the number of original bacteria is 1.0×10 4 cfu / ml; ferment at 35°C for 24 hours to make the number of bacteria >6.0×10 9 cfu / ml;

[0047] (3) Bacteria collection: collect the lactic acid bacteria fermentation liquid, centrifuge at 7500r / min, 4°C for 4 minutes, discard the supernatant and collect the bacterium sediment;

[0048] (4) Freeze-drying of bacteria slime: add freeze-drying protective agent and water to the precipitate collected in step (3), 80% of bacteria slime, 1% of trehalose, 18% of water, the thickness of the plate is 1.0cm, -75°C Freeze overnight, and vacuum freeze-dry for 18 hours with a vacuum freeze dryer to obtain a semi-finished freeze-dried powder. T...

Embodiment 3

[0051] (1) Preparation of medium: dissolve 10 g of peptone, 5 g of yeast extract, 20 g of glucose and 1.5 mL of Tween 80 in 1000 mL of distilled water, and sterilize at 122°C for 20 minutes;

[0052] (2) Activation of lactic acid bacteria: insert Bifidobacterium longum BL986 into the sterilized medium, so that the number of original bacteria is 1.0×10 4 cfu / ml; ferment at 40°C for 60 hours to make the number of bacteria >3.0×10 9 cfu / ml;

[0053] (3) Bacteria collection: collect the lactic acid bacteria fermentation liquid, centrifuge at 6000r / min, 4°C for 8 minutes, discard the supernatant and collect the bacterium sediment;

[0054] (4) Freeze-drying of bacteria slime: add freeze-drying protective agent and water to the precipitate collected in step (3), 90% of bacteria slime, VC0.3%, 0.8% of soybean lecithin, 8.9% of water, and the thickness of the plate is 2.0% cm, frozen overnight at -80°C, vacuum freeze-dried for 24 hours with a vacuum freeze dryer to obtain a semi-fin...

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Abstract

The invention discloses a bifidobacterium longum BL986, freeze-dried powder prepared from the same and application of the freeze-dried powder. The bifidobacterium longum BL986 was submitted to the China General Microbiological Culture Collection Center (CGMCC) on October 28, 2019 for preservation with the preservation number CGMCC No.18743. When the bifidobacterium longum BL986 is used to preparelactic acid bacteria freeze-dried powder and the prepared lactic acid bacteria freeze-dried powder is used to prepare chocolate, the dispersibility is good, the uniformity is high, and the product quality is greatly improved.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a bifidobacterium longum freeze-dried powder, a preparation method and an application thereof. Background technique [0002] Under the catalysis of high-intensity work, the "snackization" of catering has become one of the most important development trends in the global food market. According to a survey, about one-third of Britons eat two or more snacks at work every day. The growing "snackization" of consumption has become one of the potential markets for functional ingredients. However, over-refined food, irregular eating habits, strong life pressure and fast-paced life have caused a certain group of people to have weak spleen and stomach. [0003] As the chocolate market matures, consuming chocolate as a high-fat product is now viewed as a sweet liability. Therefore, adding probiotics represented by lactic acid bacteria to chocolate will be a new development trend. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23G1/42A23G1/40A23G1/36A23G1/46A23G1/32C12R1/01
CPCC12N1/20A23G1/423A23G1/426A23G1/40A23G1/46A23G1/36A23G1/32A23V2002/00C12R2001/01C12N1/205A23V2400/533A23V2200/3204A23V2250/61A23V2300/10A23V2300/20A23V2250/636A23V2250/642A23V2250/18
Inventor 杨锁华姜益军何卫星高瑜璘杨阳潘淑凌
Owner SYNBIOTECH BIOTECHNOLOGY YANGZHOU CO LTD
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