Application of desmethylisobordine in preparation of medicament for preventing and/or treating bladder smooth muscle dysfunction
A technology of desmethylisoboldine and dysfunction, which is applied in the field of bladder smooth muscle dysfunction treatment, can solve the problems of anticholinergic drugs limiting clinical application and unsatisfactory curative effect, and achieve the goal of reducing contractility, reducing contraction, and good regulation Effect
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Embodiment 1
[0033]The effect of desmethylisoboldine on the contractility of detrusor muscle
[0034]Take the mouse bladder, use a scalpel to cut the bladder into 4 parts (each part is 5~10mm×1~5mm), and tie the two ends of each part into a circle with No. 6 surgical thread, and one end is attached to the fixed part. On the hook, the other end is connected to a tension sensor to measure tension (HarvardApparatus, USA). The tissue is immersed in an organ bath containing Kreb's solution. The solution is maintained at 37°C and oxygen is continuously supplied. The gas composition is as follows: 950mL / LO2+50mL / L CO2. After adapting to a tension-free state for 0.5h, stretch the bladder tissue to a tension of 0.5g and balance for 1h. The changes in tension were displayed on the physiological recorder (Harvard Apparatus, USA & LabChart, New Zealand), and the data was recorded by the software (LabChart Software).
[0035]After equilibrium, EFS (40V, 2-30Hz, 0.5ms pulse duration, 10s) caused nerve-mediated cont...
Embodiment 2
[0037]Effect of desmethylisoboldine on contractility of mouse bladder smooth muscle (DSM)
[0038]1) Take the mouse bladder, use a scalpel to cut the bladder into 4 parts (each 5-10mm×1-5mm), and tie the two ends of each part into a circle with No. 6 surgical thread, and attach one end to it. On a fixed hook, the other end is connected to a tension sensor to measure tension (HarvardApparatus, USA). The tissue is immersed in an organ bath containing Kreb's solution. The solution is maintained at 37°C and oxygen is continuously supplied. The gas composition is introduced As follows: 950mL / LO2+50mL / L CO2. After adapting to a tension-free state for 0.5h, stretch the bladder tissue to a tension of 0.5g and balance for 1h. The tension change is displayed on the graph recorder (HarvardApparatus, USA & LabChart, New Zealand), and the data is recorded by the software (LabChartSoftware). After the tension is balanced, observe the contractile ability of the mouse DSM to α,β-methylene ATP (100μmol...
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