Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of gold nanoclusters in detection of protein amyloid fibrosis and screening of inhibitors in biological samples

A technology of biological samples and gold nanoclusters, which is applied in the field of protein amyloid fibrillation detection, can solve the problems of cumbersome synthesis methods, and achieve the effects of high sensitivity, large Stokes shift, and low toxicity

Active Publication Date: 2022-07-08
SHAANXI NORMAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When they are combined with protein fibers, their fluorescence intensity will increase, and with the increase of protein incubation time, the fluorescence intensity will tend to remain unchanged until the end of the fibrosis process, but these methods are still Small molecule probes, their synthesis methods are relatively cumbersome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of gold nanoclusters in detection of protein amyloid fibrosis and screening of inhibitors in biological samples
  • Application of gold nanoclusters in detection of protein amyloid fibrosis and screening of inhibitors in biological samples
  • Application of gold nanoclusters in detection of protein amyloid fibrosis and screening of inhibitors in biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Application of gold nanoclusters in the detection of protein amyloid fibrosis in biological samples

[0028] Taking lysozyme as the model protein, the specific detection method is as follows:

[0029] 1. Accurately weigh 2 mg of lysozyme and 0.0176 g of NaCl into a centrifuge tube, then add 2 mL of pH=2 hydrochloric acid solution to it and shake it to fully dissolve it, then add 20 μL of 5 mM NaN to it 3 Aqueous solution, make lysozyme, NaCl, NaN 3 The final concentrations were 1 mg / mL, 150 mM, and 0.05 mM, respectively. Finally, the centrifuge tube was sealed with parafilm, placed in a shaking water bath at 65° C., shaking at a speed of 60 rpm, and incubated for 120 h to make the lysozyme completely fibrillated. After the lysozyme fibrosis process was completed, the lysozyme fiber samples were taken out and stored in a 4°C refrigerator.

[0030] To demonstrate that gold nanoclusters can be used to detect lysozyme fibrosis, a control experiment was first performed. ...

Embodiment 2

[0041] Application of gold nanoclusters in screening inhibitors of protein amyloid fibrosis

[0042] Using lysozyme as a model protein, the inhibitory effect of ascorbic acid on protein fibrosis was studied. The specific methods are as follows:

[0043] 1. Incubate lysozyme according to the method in step 1 of Example 1 above, and add 40 μL of samples to 410 μL of ultrapure water at 0h, 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, and 120h respectively. , and then add 50 μL of gold nanoclusters to the sample. The excitation wavelength was set to 365 nm, and the scanning wavelength range was 500-700 nm to detect the fluorescence of each sample. The fibrosis process of lysozyme was detected by the fluorescence response of gold nanoclusters to lysozyme at different incubation times. Taking the incubation time as the abscissa and the fluorescence intensity of the gold nanoclusters as the ordinate, the growth curve of the fluorescence intensity with the incubation time was plott...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of gold nano-clusters in detecting protein amyloid fibrosis and screening inhibitors in biological samples. In the present invention, lysozyme is used as a model protein, and it is found that the fluorescence intensity of gold nanoclusters increases with the increase of the concentration of lysozyme fibers, and the concentration of lysozyme fibers has a linear relationship with the fluorescence intensity within a certain concentration range, so it can be Application of gold nanoclusters for the detection of protein amyloid fibrosis processes in biological samples. Compared with the fluorescence method using the traditional organic dye thioflavin T (ThT), gold nanoclusters have the advantages of low toxicity and large Stocks shift, and the fluorescence signal of gold nanoclusters is sensitive to the degree of protein fibrosis, which can be detected more sensitively. The transition of proteins from the initial stage of fibrosis to the logarithmic growth phase can more effectively study the process and mechanism of protein amyloid fibrosis, and can be used to screen inhibitors of protein amyloid fibrosis.

Description

technical field [0001] The invention belongs to the technical field of protein amyloid fibrosis detection, in particular to the application of gold nanoclusters in detecting protein amyloid fibrosis in biological samples, and the application of gold nanoclusters in screening protein amyloid fibrosis inhibitors. Background technique [0002] Elderly diseases such as Alzheimer's disease and Huntington's disease have attracted more and more attention. Clinical studies have shown that an important cause of these geriatric disorders is protein amyloid fibrosis due to denatured aggregation of proteins in the body. For example, type 2 diabetes occurs when insulin denatures and accumulates in the body. Therefore, it is of great significance to study the mechanism of protein fibrosis and to screen inhibitors of protein amyloid fibrosis in the treatment and prevention of diseases. However, the formation mechanism of amyloid fibrils is still very controversial, and a variety of forma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 张耀东郭英马雪娟夏梦凡蒲莉孙鹏跃
Owner SHAANXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com