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A kind of wolfberry polysaccharide, preparation method and application thereof

A technology of Lycium barbarum polysaccharides and Lycium barbarum, applied in the field of preparation of anticancer drugs, achieving excellent results

Active Publication Date: 2021-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of polysaccharides with RGI structure from Lycium barbarum and its application in cancer treatment drugs.

Method used

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  • A kind of wolfberry polysaccharide, preparation method and application thereof
  • A kind of wolfberry polysaccharide, preparation method and application thereof
  • A kind of wolfberry polysaccharide, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of Lycium barbarum polysaccharide, comprising the steps of:

[0027] (1) Lycium barbarum pretreatment: dry the wolfberry in an oven at 65°C and pulverize it with a pulverizer, soak the pulverized powder in acetone for 3 hours to degrease, filter and soak in 80wt% ethanol for 3 hours to remove free sugar, filter And after volatilizing ethanol in the residue, dry it.

[0028] (2) Polysaccharide extraction: the obtained wolfberry residue was stirred and extracted with 1:30 0.6wt% NaOH solution at 32°C for 10 minutes, extracted twice, and the combined extract was cooled and filtered with a 500-mesh filter cloth, and the filtrate was reused. A Sigma (3K15) centrifuge was centrifuged at a speed of 8000r / min for 10 minutes at 4°C, and the obtained supernatant was adjusted to a pH of 6-7, and 3 times the volume of the extract was added with 95wt% ethanol, and allowed to stand at 4°C for 24 After one hour, the precipitate was collected and washed three time...

Embodiment 2

[0030] (1) Lycium barbarum pre-treatment: dry the wolfberry in a 60°C oven and pulverize it with a pulverizer, soak the pulverized powder in acetone for 2.5 hours to degrease, filter and soak in 85wt% ethanol for 2.5 hours to remove free sugar, filter And after volatilizing ethanol in the residue, dry it.

[0031] (2) Polysaccharide extraction: the obtained wolfberry residue was stirred and extracted with 1:20 0.6wt% NaOH solution at 40°C for 20 minutes, extracted twice, and the combined extract was cooled and filtered with 800 mesh filter cloth to obtain the above The clear solution was adjusted to a pH of 6-7 with hydrochloric acid, and 3 times the volume of absolute ethanol was added to the extract, and the precipitate was collected after standing at 4°C for 12 hours, washed with absolute ethanol and acetone for 3 times in turn, and after air-drying, the The precipitate was redissolved in water, and then dialyzed against deionized water for two days with a dialysis bag with...

Embodiment 3

[0033] (1) Lycium barbarum pre-treatment: dry the wolfberry in an oven at 55°C and pulverize it with a pulverizer, soak the pulverized powder in acetone for 3 hours to degrease, filter and soak in 80wt% ethanol for 3 hours to remove free sugar, filter And after volatilizing ethanol in the residue, dry it.

[0034] (2) Polysaccharide extraction: the obtained wolfberry residue was stirred and extracted with 1:30 0.6wt% NaOH solution at 32°C for 10 minutes, extracted once, and the extract was cooled and filtered with an 800-mesh filter cloth to obtain the supernatant Adjust the pH to 6-7, add 3 times the volume of absolute ethanol to the extract, collect the precipitate after standing at 4°C for 12 hours, wash the precipitate with absolute ethanol and acetone alternately for 3 times, and redissolve the precipitate in water, and then use a dialysis bag with a molecular cut-off of 10,000 Da to dialyze against deionized water for two days, and finally dry the polysaccharide solution...

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Abstract

The invention discloses a Lycium barbarum polysaccharide, a preparation method and an application thereof. The Lycium barbarum polysaccharide is prepared by an alkali extraction method; the monosaccharide composition of the Lycium barbarum polysaccharide LALP includes arabinose, galactose, galacturonic acid, rhamnose, and mannose Sugar, glucuronic acid, glucose and fucose, of which the ratio of arabinose, galactose, galacturonic acid, rhamnose, mannose, glucuronic acid, glucose and fucose is 37.26:22.23:14.53: 7.66:4.11:1.61:6.18:6.43. The polysaccharide LALP contains two main components, the molecular weights of the two components are 183.9kDa and 316kDa, the polydispersity coefficients are 2.793 and 1.411, and the molecular gyration radii are 17.5nm and 37.3nm, respectively. Through structural analysis, it is found that it is mainly composed of pectin polysaccharides with RG‑I structure. Experiments show that the above polysaccharides can be well combined with Gal‑3, the molecular target of anticancer therapy. The binding constant is lower than that of other types of plant polysaccharides, and the effect is excellent. , can be developed into carbohydrate drugs for the treatment of cancer.

Description

technical field [0001] The present invention relates to polysaccharides, their extraction methods and their applications in the field of medical technology, more specifically, to a method for extracting polysaccharides rich in RG-I structure from Lycium barbarum and its application in the preparation of anticancer drugs Applications. Background technique [0002] Plant polysaccharides are ubiquitous in plants in nature, and are one of the four basic substances that constitute life activities, and are closely related to life-sustaining functions. It has a variety of functional activities such as anti-tumor, anti-coagulation, anti-aging, hypoglycemic and immunomodulatory activities, and has attracted widespread attention. [0003] Ningxia wolfberry (Lycium barbarum L.) is a perennial deciduous shrub of the genus Lycium barbarum in the family Solanaceae. The fresh fruit is a small red berry, sweet in taste and flat in nature. In traditional Chinese medicine, wolfberry is reg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715A61P35/00C08B37/0003C08B37/006
Inventor 周声怡叶兴乾陈士国陈健乐沈欣怡
Owner ZHEJIANG UNIV
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