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Method for preparing Deinagkistrodon acutus polypeptide through enzymolysis

A technology of enzymatic hydrolysis and alkaline enzyme, which is applied in the field of enzymatic hydrolysis and preparation of Qi snake polypeptide, can solve the problem that the polypeptide is difficult to effectively decompose and dissolve, and achieve the effect of increasing diversity and overall content

Inactive Publication Date: 2020-04-17
黄山市三祈生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for enzymatic hydrolysis to prepare Qi snake polypeptide, which solves the problem that the polypeptide is difficult to effectively decompose and dissolve in the traditional water extraction and wine extraction process

Method used

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  • Method for preparing Deinagkistrodon acutus polypeptide through enzymolysis
  • Method for preparing Deinagkistrodon acutus polypeptide through enzymolysis
  • Method for preparing Deinagkistrodon acutus polypeptide through enzymolysis

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Embodiment 1

[0017] Embodiment 1: A kind of method for enzymatic hydrolysis to prepare Qi snake polypeptide, comprising the following steps:

[0018] 1) Peel the freshly slaughtered Qi snake, remove the internal organs, wash and drain, put it in a meat grinder and grind it into minced meat;

[0019] 2) Put the minced meat in a container, and add water 2.5 times the weight of the minced meat into the container, stir evenly to obtain a mixed solution, let the mixed solution stand for 2 to 2.5 hours, and then process it under the action of Qi snake endogenous enzymes. enzymolysis;

[0020] 3) Heat the mixed solution in step 2) to 45-55°C, adjust the pH value of the mixed solution to PH5.5, then add papain to the container and let stand for enzymatic hydrolysis for 1.5-3 hours. Described papain enzyme activity is 100,000 u / g, and the add-on of described papain is 2~4g / 100ml to add according to the volume ratio with mixed solution;

[0021] 4) After the enzymolysis in step 3) is completed, ad...

Embodiment 2

[0025] The effects of the addition amount of various enzymes and enzymatic hydrolysis time on the polypeptide content are analyzed through experiments below.

[0026] 1 material

[0027] 1.1 Materials and reagents

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Abstract

The invention discloses a method for preparing a Deinagkistrodon acutus polypeptide through enzymolysis. The method comprises the following steps: peeling freshly slaughtered Deinagkistrodon acutus, removing internal organs, carrying out cleaning and draining, putting the treated meat of Deinagkistrodon acutus into a meat grinder, and conducting stirring to obtain meat paste; putting the meat paste into a container, adding water with a weight 2.5 times the weight of the meat paste into the container, carrying out uniform stirring to obtain a mixed solution, and subjecting the mixed solution tostanding for 2-2.5 hours; and then successively adding papain, bacillus subtilis neutral protease and alkaline enzyme into the mixed solution for enzymolysis so as to cleave fragments containing richactive proteins in the Deinagkistrodon acutus into micro-molecular polypeptide which is higher in activity and easier to absorb. The method can significantly improve the dissolution rate of the polypeptide in the meat of Deinagkistrodon acutus, and has high application value in the fields of medicinal use of Deinagkistrodon acutus and preparation of the polypeptide.

Description

technical field [0001] The invention relates to the technical field of animal polypeptide decomposition, in particular to a method for enzymatically preparing the snake polypeptide. Background technique [0002] Qi snake (Viper) is a commonly used traditional Chinese medicine, which was first recorded in "Kaibao Materia Medica". Qi snake meat is warm and salty in nature, enters the liver meridian, has a small poison, and has the effects of dispelling rheumatism, relaxing tendons and collaterals, relieving convulsions, and attacking toxins. Modern medical research has proved that the main active ingredients of Qi snake are proteins, amino acids, phospholipids, nucleosides, steroids, polypeptides, and minerals. The water extract of Qi snake can activate the fibrinolytic system; the alcohol extract can enhance the phagocytosis of macrophages and significantly increase the clearance rate of charcoal particles. [0003] At present, there are as many as 30 commercially available...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 孔天翰汪胜松李红艳汪振兴王涛廖国奇
Owner 黄山市三祈生物医药科技有限公司
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