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Preparation method of culture medium for organic fertilizer production

A culture medium and organic fertilizer technology, applied in chemical instruments and methods, mixers, shaking/oscillating/vibrating mixers, etc., can solve problems such as unfavorable liquid organic fertilizer production, affecting fermentation speed, etc. Water flow, the effect of increasing the cultivation area

Pending Publication Date: 2020-04-17
重庆酷熊科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of actually cultivating yeast, it is found that less yeast is produced in the medium in the culture dish per unit time, which will affect the fermentation speed of raw materials such as animal waste, which is not conducive to the production of liquid organic fertilizer

Method used

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  • Preparation method of culture medium for organic fertilizer production
  • Preparation method of culture medium for organic fertilizer production
  • Preparation method of culture medium for organic fertilizer production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the culture medium that is used for organic fertilizer production, comprises the steps:

[0032] Step 1: Prepare raw materials and medium preparation equipment, raw materials include beef extract 30g, peptone 15g, sodium chloride 45g, agar 150g, eggs 200g (five), oyster shells 300g (two), and the balance is water.

[0033] Such as figure 1 The medium preparation equipment shown includes a frame, a cylinder (not shown) and a mixing mechanism. The cylinder is fixed on the frame by bolts, and the upper end of the output shaft of the cylinder is welded with a support plate, which is used to support the culture medium. Dish 1, the length and width of the petri dish are both 10cm. Such as figure 2 As shown, the mixing mechanism includes an I-shaped bar 2, a T-shaped bar 3, a T-shaped tube and a sliding tube 4. The lower end of the I-shaped bar 2 is fixed on the frame by bolts, and the right end of the T-shaped bar 3 is welded with a limit block 5....

Embodiment 2

[0048] The difference from Example 1 is that the raw materials prepared in step 1 include 20g of beef extract, 10g of peptone, 40g of sodium chloride, 140g of agar, 160g of eggs (four), 150g of oyster shells (one), and the balance is water .

Embodiment 3

[0050] The difference from Example 1 is that the raw materials prepared in step 1 include beef extract 40g, peptone 20g, sodium chloride 60g, agar 160g, eggs 240g (six), oyster shells 450g (three), and the balance is water.

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Abstract

The invention relates to the field of organic fertilizer production, in particular to a preparation method of a culture medium for organic fertilizer production. The preparation method comprises the following steps: step 1, preparing raw materials and preparation equipment; step 2, adding the raw materials and carrying out primary positioning on the adding amount of water; 3, mixing the raw materials, and allowing the raw materials to be attached onto eggs; 4, dispersing agglomerated raw materials; 5, positioning the adding amount of the water again; 6, putting oyster shells into a culture dish, and coating the oyster shells with the raw materials; and step 7, sterilizing the culture dish. When such a technical scheme is adopted, the culture speed of strains can be increased in the preparation process of the culture medium.

Description

technical field [0001] The invention relates to the field of organic fertilizer production, in particular to a preparation method of a culture medium for organic fertilizer production. Background technique [0002] The nutrients of liquid organic fertilizers come from animals, plants or minerals. The raw materials for the production of organic fertilizers include animal leftovers. By hydrolyzing the protein in the leftovers, proteinaceous organic nitrogen is converted into water-soluble organic nitrogen and available nitrogen. [0003] At present, the microbial raw material commonly used to hydrolyze protein is yeast. Yeast can produce protease, so that protein can be effectively decomposed by protease. Therefore, in the production process of liquid organic fertilizer, yeast is very important for the decomposition of protein. [0004] Yeast is usually cultured with medium now, and there are many types of medium. Commonly used medium is composed of beef extract, peptone, sodi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16B01F11/00
CPCC12N1/16B01F31/443
Inventor 张巍刘存寿叶光忠梁涛罗金
Owner 重庆酷熊科技有限公司
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