Overall planning method for large-scale glutinous wheat grain identification

Abstract

The invention relates to an overall planning method for large-scale glutinous wheat grain identification. The method comprises the steps: transversely cutting wheat grains and enabling the section tobe away from a germ end; carrying out iodine-potassium iodide solution light dyeing on the cross section of the germ end, sucking up a dyed solution floating on the surface of the germ end, and then carrying out drying; and carrying out color identification on the dried germ end through professionals, reserving glutinous grains, directly sowing the dyed germ ends, and carrying out later agronomictrait investigation. According to the overall planning method for identifying the large-scale glutinous wheat grains, the preservation space of the wheat grains is greatly saved, and errors caused bydisordered control of the wheat grains are avoided; the color display is facilitated, and the color development error is reduced; and the drying treatment can enable the color identification to be more convenient, the storage of the germ end is more convenient, and the condition of seed germination or seed rotting caused in the storage process is reduced, so that the identification efficiency of theglutinous wheat is greatly improved.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to an overall planning method for large-scale identification of waxy wheat grains. Background technique [0002] At present, the identification methods of waxy wheat mainly include iodine staining, SDS-PAGE gel electrophoresis identification and Wx gene molecular marker identification. SDS-PAGE gel electrophoresis identification and Wx gene molecular marker identification methods must be completed by scientific and technical personnel with certain experimental technical skills and laboratories with comparable experimental conditions, and the grain staining method can be directly planted after the waxy grains are identified , become a waxy plant, but the Wx gene molecular marker identification method needs to be identified at the seedling stage, which increases the workload. Iodine staining is divided into ordinary staining and improved iodine staining. Ordinary i...

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Problems solved by technology

However, the ordinary grain dyeing method and the improved method both dye the far embryo end of the grain, and then carry out identification. In this way, the embryo end and the far embryo end need to be preserved at the same time, which takes up a lot of space, and the number of grains that need to be identified is large. In some cases, it is particularly labor-intensive and time-consuming, which is not conducive to large-scale operations, and it is particularly prone to errors when one seed is stored in two places; and the color difference is not obvious due to the unevenness of the dyed iodine solution, which leads to the difference in the observation and distinction. Difficulty

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