Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compositions for use for treating cutaneous leishmaniasis

A leishmaniasis and composition technology, applied in the field of cutaneous leishmaniasis, can solve the problems of high incidence of side effects of pentavalent antimony-containing agents, hindering therapeutic application, host biological toxicity, etc.

Inactive Publication Date: 2020-04-10
BIONOOX SA
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pentavalent antimony-containing agents have a high incidence of side effects
However, this class of compounds has non-negligible toxicity to host organisms, which hinders their therapeutic applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compositions for use for treating cutaneous leishmaniasis
  • Compositions for use for treating cutaneous leishmaniasis
  • Compositions for use for treating cutaneous leishmaniasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0277] Embodiment 1: the composition of the present invention application

[0278] The composition comprising DHQ (dihydroquercetin), bisabolol, optionally alpha-tocopherol and a cosmetically and pharmaceutically acceptable carrier (oil-in-water emulsion) is given in Table 1 below.

[0279] Table 1

[0280]

[0281] The oil-in-water emulsion used as carrier in the composition of Table 1 had the following composition:

[0282] water;

[0283] sweet almond oil;

[0284] Caprylic / capric triglycerides;

[0285] Olus oil;

[0286] avocado oil;

[0287] ethanol;

[0288] cetearyl alcohol;

[0289] Dioctyl ether;

[0290] glycerin;

[0291] Propylene glycol;

[0292] Sorbitol;

[0293] Glyceryl Stearate;

[0294] Glyceryl Citrate Stearate;

[0295] Polyglyceryl-3 Methyl Glucose Distearate;

[0296] xanthan gum;

[0297] Sodium dehydroacetate;

[0298] sodium benzoate;

[0299] phenoxyethanol; and

[0300] citric acid.

Embodiment 2

[0301] Embodiment 2: Anti-Leishmania activity in vitro

[0302] The anti-Leishmania activities of dihydroquercetin, ethyl 3-(2-chloroacetamido)benzoate and bisabolol were evaluated.

[0303] Compounds were evaluated for direct microbicidal action against Leishmania parasites in infected macrophages and neutrophils.

[0304] Bone marrow-derived macrophages (BMDM) were cultured for 7 days, collected, and infected with Leishmania major mCherry for 2 hours. Non-phagocytosed parasites were then washed away and dihydroquercetin, ethyl 3-(2-chloroacetamido)benzoate or bisabolol were added at different concentrations and incubated at different time points.

[0305] - Evaluation of parasiticidal efficacy by flow cytometry and microscopy.

[0306] - NO production was assessed by Griess reaction of the supernatant.

[0307] Neutrophils were isolated by magnetic activated cell sorting (MACS) and infected with Leishmania major mCherry at a multiplicity of infection (MOI) of 1:10.

[...

Embodiment 3

[0313] Example 3: In Vitro Toxicity Evaluation of Individual Components

[0314] with dihydroquercetin (5, 15 and 25 μM), ethyl 3-(2-chloroacetamido)benzoate (1, 2 and 4 μM) and bisabolol (0.05%, 0.1% and 0.5% w / v) Incubate for 24 hours, stain with DAPI, and assess neutrophil viability by flow cytometry.

[0315] Dihydroquercetin and bisabolol showed no cytotoxicity.

[0316] However, ethyl 3-(2-chloroacetamido)benzoate resulted in decreased neutrophil viability at the concentrations evaluated.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the use of a composition comprising dihydroquercetin, alpha-tocopherol, and optionally bisabolol and / or a compound of formula (I) for use in the treatment of leishmaniasis, especially cutaneous leishmaniasis. The invention further relates to the use of such a composition for treating inflammation induced by leishmaniasis, especially cutaneous leishmaniasis. The invention also relates to a method for treating leishmaniasis, especially cutaneous leishmaniasis, and / or the associated inflammation by administering an effective amount of a composition comprising dihydroquercetin, bisabolol, alpha-tocopherol and optionally bisabolol and / or a compound of formula (I) to a subject in need thereof.

Description

technical field [0001] The present invention is in the field of treatment of leishmaniasis, in particular cutaneous leishmaniasis. [0002] In particular, the present invention relates to a composition comprising dihydroquercetin and alpha-tocopherol for the treatment of leishmaniasis, in particular for the topical treatment of cutaneous leishmaniasis. The composition of the invention may also comprise bisabolol and / or a compound of formula (I), such as ethyl 3-(2-chloroacetamido)benzoate or ethyl 4-(2-chloroacetamido)benzoate . [0003] The present invention also relates to a method of treating a subject in need thereof infected with a leishmaniasis parasite, said method comprising administering to said subject a composition comprising dihydroquercetin and alpha-tocopherol . Background technique [0004] Leishmaniasis is a parasitic disease transmitted by the bite of an infected sandfly. Leishmaniasis is endemic in 88 countries in Africa, Asia, Europe, and North and Sou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/351A61K31/045A61P33/02A61K31/355A61K31/245
CPCA61K31/045A61K31/245A61K31/355A61P33/02Y02A50/30A61K31/351A61K2300/00
Inventor E·斯塔尔科曼
Owner BIONOOX SA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com