Microfluidic channel carrier structure for large-scale culture of stem cells and immune cells

Abstract

The invention discloses a microfluidic channel carrier structure for large-scale culture of stem cells and immune cells. The microfluidic channel carrier structure includes a plurality of microspheres(1). The diameter of the microspheres (1) is 0.5 mm to 2.7 mm. The microspheres (1) are stacked in a fixed bed bioreactor (2) to form a fixed bed (3), and the fixed bed (3) forms a microfluidic channel carrier structure. The maximum linear space dimension L between adjacent microspheres (1) in the microfluidic channel carrier structure is 0.183 mm to 0.988 mm. The microfluidic channel carrier structure of the invention is formed by stacking a plurality of microspheres. The microfluidic channel in the microfluidic channel carrier structure can provide cells with a micro-environment closer to that in human tissues, facilitate the interaction and signal transmission between cells and between cells and extracellular matrix, facilitate the expansion of cells, and can expand the number of cellscultured simultaneously to 100-200 billion.

Description

technical field [0001] The invention relates to the technical field of stem cell and immune cell culture, in particular to the technical field of large-scale production of stem cells and immune cells, in particular to a microfluidic carrier structure for large-scale production of stem cells and immune cells. Background technique [0002] Traditional fixed bed reactors generally use internal circulation. During the internal circulation process, the medium agitator cannot know the flow rate of the medium passing through the carrier, so it is difficult to effectively control the nutrient transfer and shear force of stem cells, making the cultivation of stem cells It is difficult to improve the quality and to carry out mass production. At present, there are two types of agitation for fixed bed reactors, one is upper agitation and the other is lower agitation, but either way, there is only one agitation paddle and all use spherical heads, the agitation paddle is located mainly by...

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Problems solved by technology

[0002] Traditional fixed bed reactors generally use internal circulation. During the internal circulation process, the medium agitator cannot know the flow rate of the medium passing through the carrier, so it is difficult to effectively control the nutrient transfer and shear force of stem cells, making the cultivation of stem cells Difficult to improve quality and difficult to mass-produce

At present, there are two types of agitation for fixed bed reactors, one is upper agitation and the other is lower agitation, but either way, there is only one agitating paddle and all use spherical heads, the agitating paddle is located mainly by In the medium mixing tank, the stirring paddle stirs the medium in the medium mixing tank to make it uniform, but the internal circulation of the stirring paddle cannot know the flow rate of the carrier when the medium circulates, so that the rotation speed of the stirring paddle is related to the flow rate of the medium. The flow rate of the carrier cannot be matched, and the rotation speed of the stirring paddle cannot be adjusted; in addition, due to the large volume of the spherical head, a large amount of digestive juice remains, which affects the next growth of adherent cells, resulting in waste and low efficiency

The applicant has independently developed a fixed-bed bioreactor system equipped with a disc-shaped head, an external circulation mechanism, and dual stirring paddles. This system can simultaneously cultivate tens of billions of cells; but in actual use, the applicant found that obstacles The factor for increasing the culture capacity of this system is the carrier structure, so it is necessary to develop a new carrier structure to continue to increase the culture capacity of the fixed bed bioreactor system

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