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Grape VyCYP89A2 gene and encoding protein thereof as well as application of grape VyCYP89A2 gene in drought-resistant variety breeding

A technology for encoding proteins and grapes, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as unreported

Active Publication Date: 2020-03-17
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CYP86 in P450 is a newly discovered family consisting of 4 subfamilies. The genes of this family mostly encode fatty acid hydroxylases, which are involved in the catabolism of fatty acids and are closely related to plant growth and development and defense against abiotic stress. The research on the function of CYP86 family genes has not been reported yet, and further exploration is needed. It is expected to find genes related to grape growth and resistance to abiotic stress, which will provide new ideas for genetic improvement of grape stress resistance

Method used

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  • Grape VyCYP89A2 gene and encoding protein thereof as well as application of grape VyCYP89A2 gene in drought-resistant variety breeding
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  • Grape VyCYP89A2 gene and encoding protein thereof as well as application of grape VyCYP89A2 gene in drought-resistant variety breeding

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Experimental program
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Effect test

Embodiment 1

[0016] Example 1 Grape VyCYP89A2 gene expression analysis

[0017] After subculture of Yanshan grape tissue culture seedlings for 16 days, the seedlings with consistent growth and performance were selected for various stress treatments.

[0018] Drought treatment: Pull out the grape seedlings from the culture medium, place them on filter paper and expose them to a room temperature of (32±1)°C, a relative humidity of 55%, and a photoperiod of light 14h / darkness 10h. 2, 6, 12, 24h sampling.

[0019] Low temperature treatment: culture the tissue cultured seedlings at a temperature of (4±1)°C, a relative humidity of 75%, and a photoperiod of 14 hours of light / 10 hours of darkness, and take samples at 0, 2, 6, 12, and 24 hours.

[0020] Salt stress: add 20mL 100mmol·L -1 The NaCl solution was cultivated at a temperature of (25±1)°C, a relative humidity of 75%, and a photoperiod of 14 h light / 10 h dark, and samples were taken at 0, 2, 6, 12, and 24 h.

[0021] An equal volume of ...

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Abstract

The invention, which belongs to the technical field of plant genetic engineering, relates to a grape VyCYP89A2 gene and encoding protein thereof as well as application of the grape VyCYP89A2 gene in drought-resistant variety breeding. According to the invention, an overexpression vector of the Yanshan grape VyCYP89A2 gene is transferred into arabidopsis thaliana by using the transgenic technologyof a strong promoter (cauliflower mosaic virus 35S promoter) driving principle, so that a transgenic arabidopsis thaliana plant is obtained; the experiment proves that compared with an arabidopsis thaliana plant with a transformed empty vector, accumulation of related stress resistance substances in transgenic arabidopsis thaliana and expression of drought resistance related genes are caused by overexpression of the VyCYP89A2 gene, so that the drought resistance of the transgenic plant is enhanced. The grape VyCYP89A2 gene and the recombinant expression vector of the grape VyCYP89A2 gene can be used for breeding drought-resistant varieties of plants.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the grape VyCYP89A2 gene and its encoded protein and the application of the gene in drought-resistant variety breeding. Background technique [0002] Grapes are native to western Asia and are cultivated all over the world. About 95% of grapes around the world are concentrated in the northern hemisphere. The main producing areas in China are Xiaoxian County in Anhui, Turpan and Hotan in Xinjiang, Yantai in Shandong, Zhangjiakou, Xuanhua, Hebei Changli, Dalian, Xiongyue, Shenyang in Liaoning and Lumiao Township, Minquan, Yifeng and other places in Henan. During the growth and development of grapes, water has an important impact on the yield and quality of grapes. Water shortage will easily threaten the grape industry. Under the background of water scarcity, excavating drought-resistant grape resources and studying grape drought-resistant genes are of great scient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/82A01H5/00A01H6/20
CPCC12N9/0071C12N15/8273
Inventor 余义和杨芯贤李敏张贺程王磊磊郭大龙杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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