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A special microbial agent for apple trees and its preparation method

A microbial inoculum and technology for apple trees, applied in the field of agricultural microorganisms, can solve the problems of no obvious improvement in apple taste, poor effect of apple rot disease, and no obvious increase in apple yield, so as to prevent and treat apple rot disease, increase apple yield, The effect of improving taste

Active Publication Date: 2022-02-01
保罗蒂姆汉(潍坊)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The existing microbial agents for apple trees have the following defects: (1) the effect of preventing and treating apple rot is not good; (2) the yield of apples is not significantly improved; (3) the taste of apples is not significantly improved

Method used

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  • A special microbial agent for apple trees and its preparation method
  • A special microbial agent for apple trees and its preparation method
  • A special microbial agent for apple trees and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 a kind of special microbial bacterial agent for apple tree

[0045] Bacillus amyloliquefaciens 20 parts

[0046] Paenibacillus polymyxa 18 parts

[0047] Bacillus licheniformis 10 parts

[0048] Streptomyces jingyang 10 parts

[0049] Bacillus coagulans 6 parts

[0050] Bacillus Velez 20 parts

[0051] Glomus moses 16 parts.

[0052] The Bacillus amyloliquefaciens: strain preservation number is CICC23569; the Paenibacillus polymyxa: strain preservation number is ACCC10122; the Bacillus licheniformis: strain preservation number is CICC21228; the Streptomyces Jingyang: strain The preservation number is ACCC40126; the Bacillus coagulans: strain preservation number is ACCC10229; the Glomus mosei: strain preservation number is BGCNM02A.

Embodiment 2

[0053] Embodiment 2 A kind of special microbial bacterial agent for apple tree

[0054] Bacillus amyloliquefaciens 20 parts

[0055] Paenibacillus polymyxa 20 parts

[0056] Bacillus licheniformis 18 parts

[0057] Streptomyces jingyang 12 parts

[0058] Bacillus coagulans 5 parts

[0059] 15 servings of Bacillus velesii

[0060] Glomus moses 10 parts.

[0061] The Bacillus amyloliquefaciens: strain preservation number is CICC23569; the Paenibacillus polymyxa: strain preservation number is ACCC10122; the Bacillus licheniformis: strain preservation number is CICC21228; the Streptomyces Jingyang: strain The preservation number is ACCC40126; the Bacillus coagulans: strain preservation number is ACCC10229; the Glomus mosei: strain preservation number is BGCNM02A.

Embodiment 3

[0062] Embodiment 3 A kind of preparation method of special microbial bacterial agent for apple tree

[0063] A preparation method of special microbial bacterial agent for apple trees, comprising the following steps:

[0064] Step 1, preparation of bacillus amyloliquefaciens dormant microorganism dry powder

[0065] Inoculate the activated and cultivated Bacillus amyloliquefaciens strains into the seed tank with a volume ratio of 60% fermentation medium, the volume inoculum amount is 0.5%, the culture temperature is 38°C, the stirring speed of the seed tank is 240rpm, and the culture time is 12h , the bacteria reached the logarithmic growth phase;

[0066] The cultured seed tank bacterial classification is inoculated into a fermenter with a volume ratio of 70% fermentation medium, and the volume inoculum is 5%. The formula of the fermentation medium in the seed tank and the fermenter is the same. The formula is: corn flour 32g / L, soybean meal flour 36g / L, dipotassium hydroge...

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Abstract

The invention relates to a special microbial agent for apple trees and a preparation method thereof, comprising the following bacterial species components: Bacillus amyloliquefaciens, Paenibacillus polymyxa, Bacillus licheniformis, Streptomyces Jingyang, Bacillus coagulans, and Bacillus Veles bacillus and Glomus moesei; the deposit number of Glomus moesei is BGCNM02A. The special microbial agent for apple trees can effectively prevent and treat apple rot disease, and at the same time, can significantly increase apple yield and apple sugar content.

Description

technical field [0001] The invention relates to a special microbial agent for apple trees and a preparation method thereof, belonging to the technical field of agricultural microorganisms. Background technique [0002] Apple rot disease, commonly known as rotten skin disease and stinky skin disease, is an important disease of apple trees in northern China. The disease mainly damages fruit trees that are more than 6 years old, causing tree vigor to weaken, branches to die, dead trees, and even garden destruction. The fungus spreads and overwinters on the surface of diseased bark and xylem. Conidia are produced in early spring, and when it rains, the spore horns are extruded by the pycnidium. The conidia disperse and fly over the orchard with the wind every year. After germination, they invade the tree from the lenticels, fruit stalk scars, leaf scars and various wounds, and lie latent at the infection point, making the tree generally infected with bacteria. When the bark f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/28A01N63/30A01P3/00A01P21/00C12R1/07C12R1/12C12R1/10C12R1/465C12R1/645
CPCC12N1/20C12N1/14A01N63/00
Inventor 郭芳先韩威华肖发沂刘镇王于玺
Owner 保罗蒂姆汉(潍坊)生物科技有限公司
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