Nucleic acid extraction pretreatment amplification system

Abstract

The invention discloses a nucleic acid extraction pretreatment amplification system. The system comprises a filtering device, a DNA extraction device, a uniform mixing device, a PCR amplification device and a driving device for driving a liquid to flow. The labor time is saved and the working efficiency of people is improved through a method of sequentially treating samples by different reagents.Through ingenious pipeline design, functional units such as the filtering device, the DNA extraction device for nucleic acid extraction, the uniform mixing device and the PCR amplification device areintegrated in an embedded manner, so that the overall size is remarkably reduced. The system has the advantages of being simple in structure, easy to achieve and operate, low in cost and capable of being widely used and popularized. Besides, the PCR amplification device is based on temperature circulation, so that rapid amplification and signal amplification of polymerase on nucleic acid moleculesare realized, and the detection time is greatly shortened. In addition, measurable reagents are wide in variety, for example, bacteria, viruses, fungi, chlamydia, rickettsia and the like can be rapidly detected.

Description

technical field [0001] The invention relates to the technical field of accounting, extraction and amplification, and more specifically, to a nucleic acid extraction, pretreatment and amplification system. Background technique [0002] Quantitative PCR is the most important molecular biology detection method today, which can quickly identify bacteria, viruses, fungi and other microbial types and drug-resistant mutations, and the analysis sensitivity is far higher than other detection methods; it only takes 30 to 40 minutes. It can accurately detect the number of different microbial populations, so it has important application prospects in almost all life science fields such as food testing, clinical testing, disease control, inspection and quarantine, scientific research laboratories, food safety, cosmetics testing, and environmental hygiene. [0003] The existing PCR amplification instrument mainly has the following defects: [0004] 1. The temperature cycle adopted require...

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Problems solved by technology

[0004] 1. The temperature cycle adopted requires a complex temperature control system to realize, and the technical cost is relatively high;

[0005] 2. The volume of the whole machine is relatively large, which is not suitable for rapid detection in a timely field environment;

[0006] 3. The pretreatment and preparation process of the sample is relatively complicated, and it needs to rely on a mechanical centrifugal motion system with high energy consumption and large volume.

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