Multi-row single-tube consumable and using method of consumable in magnetic rod method nucleic acid extraction and purification

Abstract

The invention discloses a multi-row single-tube consumable and a using method of the consumable in magnetic rod method nucleic acid extraction and purification. A single-tube consumable used in a current magnetic bead method nucleic acid extraction instrument limits the further improvement of mixing and lysis effects. According to the multi-row single-tube consumable provided by the invention, thetop surface is provided with a lysing chamber and a plurality of functional chambers that are equally divided into two or three rows, and the functional chambers include washing chambers, an elutionchamber and a magnetic bead placement chamber. Due to the large space size of the lysing chamber of the multi-row single-tube consumable, the uniform mixing and lysis effects can be improved through combination of high-frequency vibration of a sheath and high-speed rotation of an operating platform, when a magnetic rod moves down to adsorb magnetic beads, the operating platform can rotate at a lowspeed, so that the magnetic rod is in contact with more space in the lysing chamber to improve the adsorption effect on the magnetic beads; and when the magnetic rod adsorbs the magnetic beads at thebottom of the lysing chamber, the operating platform rotates the lysing chamber to a place where a diameter of the lysing chamber coincides with the horizontal movement direction of the sheath, so that the motion trail of the magnetic rod becomes longer, and the adsorption effect on the magnetic beads and the adsorption efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction and purification, and in particular relates to a multi-row single-tube consumable and a method for using it in nucleic acid extraction and purification by a magnetic rod method. Background technique [0002] The existing single-tube consumables are all in a single row, and the size occupied along the translation direction of the consumables is too large, so that the size of the supporting nucleic acid extraction instrument along the translation direction of the consumables is also large; while the washing chambers and elution chambers of the existing single-tube consumables The size along the direction of vertical translation of consumables cannot be designed too large, otherwise the volume of each washing chamber and elution chamber will be too large, and a large amount of washing buffer and elution buffer will be required to reach a certain height in the washing chamber and elutio...

AI Technical Summary

Problems solved by technology

However, when performing lysis, since the single-tube consumables used in the existing magnetic bead method nucleic acid extraction instruments are all in a single row, the size perpendicular to the translation direction of the consumables is limited. Well mixed and lysed reagents, the auxiliary vibration trajectory in the horizontal direction is limited, the effect of mixing and lysed needs to be improved

[0004] In addition, when single-row single-tube consumables are used, when the magnetic rod moves down in the cracking chamber to adsorb magnetic beads, due to the limited space size of the single-tube consumable, the magnetic rod is only suitable for moving in one direction in the vertical direction, even in the horizontal direction. Translate, the displacement of translation is quite small, it can be seen that the adsorption effect of magnetic beads is poor

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