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Method and device for detecting various microorganisms in food

A technology for microorganisms and target microorganisms, applied in the field of microorganism detection, can solve problems such as inaccurate test results, and achieve the effect of being convenient to use, simple and convenient to use, and making up for non-compliance

Pending Publication Date: 2020-02-18
阮雁春
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method and device for detecting various microorganisms in food, so as to solve the situation that the detection results of some of the above-mentioned grassroots work units are inaccurate due to limited conditions.

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  • Method and device for detecting various microorganisms in food
  • Method and device for detecting various microorganisms in food
  • Method and device for detecting various microorganisms in food

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.

[0025] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "above", "below", "left", "right", "inside" etc. is based on the orientation or position shown in the drawings The positional relationship is only for the convenience of describing the present invention and simplifying the description, but does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

[0026] A method for detecting multiple microorganisms in food, comprising the following steps:

[0027] S1. Sterilize the mixing dish 2 to ensure that there are no microorganisms...

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Abstract

The invention relates to the technical field of microorganism detection, and discloses a method for detecting various microorganisms in food. The method comprises the following steps: adding a diluentto a mixing dish and diluting a food sample to be tested, wherein the ratio of the sample to the diluent is 1:10; sterilizing petri dishes, and then adding different culture media for culturing a variety of target microorganisms to the differentpetri dishes, respectively; separately adding color development compounds to the petri dishes with microorganism media added; conducting aseptic liquid separation on a diluted sample diluent through liquidseparation containers, and dividing the sample diluent equally into different petri dishes; heatingthe petri dishes and keeping the petri dishes at 35-37 DEG C for culturing, wherein the culture time is 24-36h; observing the color developmentreaction in the petri dishes after culturing to determine whether the target microorganisms are present inthe petri dishes; taking out and placing the petri dishes under a microscopefor observation, and calculating the content of the target microorganisms. The method of the invention is simple to operate,thereby facilitating the operation of basic level workers.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a method and device for detecting various microorganisms in food. Background technique [0002] With the continuous improvement of people's living standards, more and more attention has been paid to food safety issues. Whether the microorganisms in food exceed the standard is an important indicator to ensure food safety. The traditional food microbiological detection is to use the laboratory culture method, that is, to cultivate colonies on the medium, and then observe the number of colonies to detect whether the content of microorganisms exceeds the standard. The test of the laboratory culture method has high requirements on the operation level of the experimenters and the equipment. In some grassroots work units, the staff's operation level is not high, and the equipment is not up to standard. Miscellaneous bacteria were mixed in the experiment, resulting in in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/04C12M1/38C12M1/36C12M1/34C12M1/12C12M1/04C12M1/02C12M1/00
CPCC12Q1/06C12Q1/04C12M23/40C12M23/48C12M29/20C12M37/00C12M41/12C12M41/20C12M41/36
Inventor 阮雁春
Owner 阮雁春
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