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Chromatographic strip comprising multiple test lines, diagnostic kit comprising same, and qualitative, semi-quantitative or quantitative analysis method comprising multiple competitive reaction measurement steps

A technology for diagnostic kits and chromatographic bands, applied in the field of chromatographic bands, can solve problems such as low detection limit and insufficient quantification

Active Publication Date: 2020-02-11
普默特株式会社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the rapid kit using this competitive reaction has the advantages of low analysis cost due to easy mass production, easy use method and rapid result reading, and does not require expensive and large-scale professional analysis equipment, but there is a detection limit ( detection limit) and the relative lack of quantification due to the fact that the naked eye is usually used to judge
For this reason, the conventional chromatographic analysis method was mainly used for qualitative analysis, and there were many limitations in its use for quantitative analysis.

Method used

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  • Chromatographic strip comprising multiple test lines, diagnostic kit comprising same, and qualitative, semi-quantitative or quantitative analysis method comprising multiple competitive reaction measurement steps
  • Chromatographic strip comprising multiple test lines, diagnostic kit comprising same, and qualitative, semi-quantitative or quantitative analysis method comprising multiple competitive reaction measurement steps
  • Chromatographic strip comprising multiple test lines, diagnostic kit comprising same, and qualitative, semi-quantitative or quantitative analysis method comprising multiple competitive reaction measurement steps

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1: the making of chromatographic dipping (dip) band

[0085] A. Fabrication of test pads with test lines

[0086] Three test lines were rametted on nitrocellulose membranes. The nitrocellulose membrane was laminated on the plastic using a laminator. After that, rabbit IgG (rabbitimmunoglobulin G, Arista, USA) was used as the target substance or analogue of the test line, and an automatic ramming machine was used to divide the test line 1, test line 2, and test line 3 at regular intervals. After branching, drying was performed for 2 days (48 hours) at 25 to 30°C.

[0087] B. Absorbent pad (absorbance production of pad)

[0088] The absorbent pad is used without any treatment in a state where moisture is completely dried in a dryer, and is prepared by cutting it into an appropriate size.

[0089] C. Production of chromatographic bands

[0090] according to Figure 5 In the structure shown, the detection pad and the absorbent pad prepared through t...

Embodiment 2

[0097] Example 2: Analysis Using Dip Bands for Chromatography

[0098] 100 μl of a sample solution containing rabbit immunoglobulin (rabbitIgG) as a target substance and phosphate buffered saline (PBS) were divided into 96 well plates, respectively. The sample solution was prepared so that the concentration of rabbit immunoglobulin was 0, 5, 10, 50, 100, 500, 1000, and 5000 ug / ml, respectively. A labeling substance (fluorescence)-first ligand (anti-rabbit immunoglobulin) solution is added to the sample solution as a first predetermined amount.

[0099] The 96-well plate containing the above-mentioned sample solution was put into the chromatographic dipping tape prepared in the above-mentioned Example 1, and developed for 10 minutes.

[0100] Image 6It is a graph showing the development results of chromatographic bands based on the concentration of rabbit immunoglobulin as a target substance.

[0101] It was confirmed that for test line 1, test line 2, and test line 3, the ...

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Abstract

The present invention relates to: a chromatographic strip comprising a plurality of test lines for a competitive assay; a diagnostic kit comprising the chromatographic strip; and a method for qualitatively or quantitatively analyzing a target material within a specimen by using the same. By using the chromatographic strip of the present invention, qualitative analysis and highly reliable quantitative analysis can be rapidly and conveniently carried out with the naked eyes or a sensor even for a target material, which has one ligand binding position or is small. Furthermore, the present invention has an effect of improving the concentration measurement range of an analyte by the mutual supplementation of test lines and control lines.

Description

technical field [0001] The invention relates to a chromatographic strip with multiple test lines, a diagnostic kit with the above-mentioned chromatographic strip and a method for qualitatively and / or quantitatively analyzing target substances in a sample by using multiple competitive reactions. The device for chromatographic analysis of the present invention can be used for qualitative, semi-quantitative, and quantitative analysis in various fields such as diagnosis and prediction, food safety testing, environmental analysis, and compound analysis. Background technique [0002] The chromatographic analysis method, which is recognized as a rapid test method, can use the binding reaction of the target substance and the ligand that specifically binds to the target substance to qualitatively and quantitatively analyze the trace amount of the target substance (analyte) in a short period of time. The method of quantitative analysis is used in various fields such as medical treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
CPCA61B5/00G01N30/90G01N33/54388A61B5/41
Inventor 林国镇金泰亨朴东奭辛硕教金美晶
Owner 普默特株式会社
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