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Advanced treatment method for antibiotic resistance genes in sewage

An antibiotic resistance and advanced treatment technology, applied in water/sewage treatment, oxidized water/sewage treatment, water/sludge/sewage treatment, etc., can solve problems such as insufficient removal capacity of ARGs, environmental safety risks, etc., and achieve efficient disinfection Bactericidal effect, convenient operation and management, and less reagent requirement

Active Publication Date: 2020-02-11
TONGJI UNIV
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  • Application Information

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Problems solved by technology

Some biological means such as biofilters and constructed wetlands have insufficient removal capacity for ARGs
Coagulation, membrane separation technology, etc., as effective physical separation methods, have good physical removal of removal and interception of microorganisms. However, these processes only transfer resistance genes from sewage to other media, which will still cause safety to the environment. risk, further treatment is required

Method used

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  • Advanced treatment method for antibiotic resistance genes in sewage
  • Advanced treatment method for antibiotic resistance genes in sewage
  • Advanced treatment method for antibiotic resistance genes in sewage

Examples

Experimental program
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Effect test

Embodiment 1

[0031] In this embodiment, the sewage to be treated is taken from the effluent of the secondary sedimentation tank of a domestic sewage treatment plant in Shanghai. The specific method includes the following steps:

[0032] Take 500mL of the effluent from the secondary sedimentation tank and put it in a beaker, put it in a magnetic stirrer, put it on the magnetic stirrer, and adjust the speed to 600rpm. Weigh different masses of potassium ferrate solids and add them into beakers to make the concentrations 2.5, 5, and 10 mg-Fe / L. Timing and stirring for 2min, keeping the rotation speed at 600rpm. Then the rotational speed was adjusted to 100 rpm, and the stirring was continued for 20 min. Turn off the agitator and allow the sewage sample to settle naturally for 30 minutes.

[0033] Take 400 mL of the supernatant by siphon method, and filter with a 0.22 μm filter membrane to retain bacteria and DNA. At the same time, filter 500mL of secondary sedimentation tank effluent with ...

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Abstract

The invention relates to an advanced treatment method of antibiotic resistance genes in sewage. Sewage containing antibiotic resistance genes is mixed and stirred with ferrate, and after sedimentation, a purified water body is obtained. The ferrate is added to the sewage, the antibiotic resistance genes in the sewage can be destroyed, iron-containing oxide and hydroxide generated in situ are usedas coagulant, and the antibiotic resistance genes, DNA fragments generated by oxidation of the ferrate and other transmissible genetic elements (such as integron) can further be removed. The advancedtreatment method can not only efficiently remove antibiotic resistance genes in water, but also greatly reduce the risk to the environment caused by treating by-products (precipitated solids). In addition, the provided removal method is simple in operation and convenient in operation and management, and required reagents are single in type and small in amount. The test results show that the advanced treatment method is good in removal effect on resistance genes widely detected in sewage, and the removal rate reaches 1.10-4.37 orders of magnitude.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to an advanced treatment method for antibiotic resistance genes in sewage. Background technique [0002] Antibiotics are one of the most important discoveries in the history of human medicine. Since their invention, they have been widely used in the fields of human medicine, animal disease prevention, and animal husbandry. However, the mass production and use of antibiotics has led to a significant increase in the content of antibiotics in environmental media. The stress of antibiotics has induced the evolution of antibiotic resistance in environmental microorganisms, resulting in the generation of resistance genes (Antibiotic resistance genes, ARGs) in organisms, which seriously threatens the ecological environment. safety and human health. The World Health Organization regards antibiotic resistance as one of the most serious environmental health problems facing mankind i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/72C02F1/52C02F101/10
CPCC02F1/5236C02F1/72C02F2101/105C02F2303/04
Inventor 孙婧严小芳倪丙杰徐秋翔朱卓然阿合卓力·肯加汗刘智涵
Owner TONGJI UNIV
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