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A method for separation and purification of antiallergic components in perilla leaves

A separation, purification and anti-allergic technology, applied in the direction of medical preparations containing active ingredients, allergic diseases, plant/algae/fungus/moss components, etc., can solve various complications, human troubles, safety hazards and other problems, Achieve the effects of short extraction time, simple and feasible method, and improved yield

Active Publication Date: 2022-01-25
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although allergic diseases do not pose the most direct threat to human health like cancer and cardiovascular disease, they can cause long-term distress to humans, cause multiple complications, and may even be life-threatening
However, most chemically synthesized antiallergic drugs have potential toxicity and great potential safety hazards. Therefore, seeking a natural low-toxic antiallergic drug has become a research hotspot in the field of food and drug safety.

Method used

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  • A method for separation and purification of antiallergic components in perilla leaves
  • A method for separation and purification of antiallergic components in perilla leaves
  • A method for separation and purification of antiallergic components in perilla leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take by weighing 10.0g perilla leaf powder in a plastic beaker, according to the solid-liquid ratio of 1:15 (kg / L), add 150mL of ethanol with a volume concentration of 70% therein, after stirring evenly, place the beaker in a high-speed dispersion On the instrument, set the speed of the instrument at 11000r / min, the temperature at 30°C, disperse for 1min, disperse 5 times, pour out the upper layer solution, collect, then add 150mL of 70% ethanol to the beaker, repeat the above dispersion process, and combine the obtained upper layer The solution was subjected to suction filtration, and the obtained clear solution was dried at 50°C to obtain a crude extract;

[0035] Mix the crude extract with silica gel (200-300 mesh) at a mass ratio of 1:1, place in a mortar, grind evenly, and obtain a sample for later use; weigh an appropriate amount of silica gel and add petroleum ether and mix well, for later use; take a Put a small amount of cotton at the bottom of the chromatograp...

Embodiment 2

[0037] Take by weighing 10.0g perilla leaf powder in a plastic beaker, add 150mL of ethanol with a volume concentration of 70% according to a solid-liquid ratio of 1:20 (kg / L), stir evenly, put the beaker into a high-speed disperser Above, set the speed of the instrument at 11000r / min, the temperature at 35°C, disperse for 1min, disperse 6 times, pour out the upper solution, collect it, then add 150mL of 70% ethanol to the beaker, repeat the above dispersion process, combine the upper solution and Suction filtration was carried out, and the obtained clear solution was dried at 50°C to obtain a crude extract;

[0038] Mix the crude extract with silica gel (200-300 mesh) at a mass ratio of 1:1, place in a mortar, grind evenly, and obtain a sample for later use; weigh an appropriate amount of silica gel and add petroleum ether and mix well, for later use; take a Put a small amount of cotton at the bottom of the chromatography column, slowly put the silica gel mixed with petroleum...

Embodiment 3

[0040]Take by weighing 10.0g perilla leaf powder in a plastic beaker, according to the solid-liquid ratio of 1:25 (kg / L), add 150mL of ethanol with a volume concentration of 70% therein, stir evenly, put the beaker into a high-speed disperser Above, set the speed of the instrument at 11000r / min, the temperature at 40°C, disperse for 1min, disperse 7 times, pour out the upper layer solution, collect it, then add 150mL of 70% ethanol to the beaker, repeat the above dispersion process, combine the upper layer solution and carry out Suction filtration, drying the obtained clear solution at 50°C to obtain a crude extract;

[0041] Mix the crude extract with silica gel (200-300 mesh) at a mass ratio of 1:1, place in a mortar, grind evenly, and set aside; weigh an appropriate amount of silica gel and add petroleum ether, mix well, set aside; take a chromatographic Plug a small amount of cotton at the bottom of the column, slowly put the silica gel mixed with petroleum ether into the ...

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Abstract

The invention discloses a method for separating and purifying anti-allergic components in perilla leaves. Firstly, fresh perilla leaves are dried in the shade at room temperature, pulverized and sieved, soaked in a solvent, and extracted by enhanced mass transfer to obtain crude anti-allergic components of perilla. Then use thin-layer chromatography to determine the elution solvent, silica gel column chromatography and Sephadex column chromatography to purify the components of the anti-allergic crude extract of Perilla perilla, and the hyaluronidase inhibition rate can be as high as 89.76 % of perilla extract with high antiallergic activity. The separation and purification method provided by the invention has the advantages of simple operation, less time consumption, high yield, remarkable activity, good reproducibility of results, etc., and is suitable for the separation and purification of most plant active substances.

Description

technical field [0001] The invention relates to the technical field of plant extract production, in particular to a method for separating and purifying antiallergic components in perilla leaves. Background technique [0002] Allergies are multiple common diseases. Although allergic diseases do not pose the most direct threat to human health like cancer and cardiovascular disease, they can cause long-term distress to humans, cause multiple complications, and may even be life-threatening. However, most chemically synthesized antiallergic drugs have potential toxicity and great potential safety hazards. Therefore, seeking a natural low-toxic antiallergic drug has become a research hotspot in the field of food and drug safety. [0003] Perilla extract has anti-allergy, anti-oxidation, lowering blood lipid and blood pressure, protecting liver, inhibiting platelet aggregation, reducing thrombosis, anti-inflammation and protecting eyesight, etc., and has high research value. Hyal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/535A61P37/08A61K127/00
CPCA61K36/535A61P37/08A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 张志军李会珍李晓君张世林陈铁
Owner ZHONGBEI UNIV
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