Rice large-grain gene function marker and application

Abstract

The invention discloses a rice large-grain gene function marker and application. The function marker is selected from one or more of a GS2 function marker, a GW2 function marker, a GL3.1 function marker and a GW2 promoter marker, wherein the GS2 function marker is a dCaps marker developed for TC / AA mutation of a third exon of a GS2 gene, the GW2 function marker is a dCaps marker developed for nucleotide A deletion mutation of a fifth exon of a GW2 gene, the GL3.1 function marker is a dCaps marker developed for single-base C / A mutation of a tenth exon of a GL3.1 gene, and the GW2 promoter marker is a dCaps marker developed for T / A mutation at upstream 858bp of a GW2 translation initiation site. The invention develops effective dCas markers for key variation sites of rice large-grain genes,can effectively identify large-grain rice germplasm and is applied to molecular breeding of rice.

Description

technical field [0001] The invention relates to rice breeding, in particular to a rice large-grain gene functional marker and application thereof. Background technique [0002] Rice is an important food crop, and its high yield determines food production security and social stability. Rice yield is mainly determined by the number of panicles, the number of grains per panicle and the thousand-grain weight. On the premise of not affecting the other two yield factors, increasing the thousand-grain weight can quickly increase rice yield; in addition, in the process of hybrid rice seed production, large-grain restorer lines can be purposefully created, and the sterile line can be bred into small-grain type. It can achieve mixed harvesting of two strains, and finally separate the large-grain and small-grain strains according to the grain weight, which can greatly reduce the cost of seed production. Therefore, cultivating large-grain rice varieties has extremely high production a...

AI Technical Summary

Problems solved by technology

Compared with sequencing, the molecular marker cost of gene PCR amplification and gel electrophoresis technology is lower, but since the three mutations all belong to base substitution or single base deletion, gel electrophoresis of PCR amplification products cannot be directly distinguished. The mutations did not produce differences in restriction sites, so PCR products cannot be used for identification. At present, there are no effective molecular markers for these three key mutations, which greatly limits the molecular breeding improvement of rice large-grain traits.

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