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Applications of 3,4-dimethylbenzaldehyde

A technology of dimethylbenzaldehyde and pest attractant, applied in the application field of 3,4-dimethylbenzaldehyde, can solve the problems of excessive pesticide residues in agricultural products and environmental pollution

Pending Publication Date: 2020-02-04
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Up to now, chemical pesticides are one of the main means of controlling plant pests. However, chemical pesticides, especially chemical pesticides, will bring environmental pollution and pest resistance, and excessive pesticide residues in agricultural products while eliminating pests.

Method used

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  • Applications of 3,4-dimethylbenzaldehyde
  • Applications of 3,4-dimethylbenzaldehyde
  • Applications of 3,4-dimethylbenzaldehyde

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] The preparation method of the pest attractant includes the preparation of mother liquid A, the preparation and preparation steps of auxiliary liquid B, specifically including:

[0023] A. Preparation of mother liquor A: Accurately weigh 3, 4-dimethylbenzaldehyde and add solvent to dissolve to prepare mother liquor A;

[0024] B. Preparation of auxiliary solution B: Prepare Tween-80 solution with deionized water to obtain auxiliary solution B;

[0025] C. Preparation: The mother liquid A and the auxiliary liquid B are mixed, then ultrasonically treated, and then constant volume is obtained to obtain the target pest attractant.

[0026] The solvent described in the A step is one or more of ethanol, n-hexane, methanol and methylene chloride.

[0027] The mass percent concentration of the Tween-80 solution described in the B step is 0.1%.

[0028] The ultrasonic treatment described in step C is ultrasonic treatment at an ultrasonic frequency of 40-60 KHz for 4-6 minutes. ...

Embodiment 1

[0036] Discovery of pest attractant substances

[0037] (1) Extraction and identification of volatile components

[0038] The volatile components of healthy and pest-free Chinese rose leaves were collected by headspace dynamic adsorption method, and the collected volatile components were stored in a -20°C refrigerator for GC-MS analysis. The adsorbent used in the collection process was Tenax TA, and the collection time was 24 hours to eliminate the influence of diurnal rate changes on the types and contents of volatile components.

[0039] (2) Feeding method of cotton bollworm

[0040] Put the collected mature larvae of cotton bollworm into a 250 mL beaker with moist sand and seal it with gauze. After pupation and eclosion, the adults were transferred to another 250 mL beaker. Supplement nutrition with 10% honey water, make them lay eggs and hatch on healthy Chinese rose leaves, and transfer them to finger tubes to raise single-headed larvae until mature (feeding conditions:...

Embodiment 2

[0049] EAG Response of Cotton Bollworm Adults to 3, 4-Dimethylbenzaldehyde

[0050] The antennae of the female moth holding eggs of cotton bollworm were cut off, and the two flagella at the end were removed, and then connected with the probe of the antenna potentiometer with conductive glue, and the antenna electrophysiology (EAG) test was performed. Take 20.0 μL of the sample solution to be tested (3, 4-dimethylbenzaldehyde-n-hexane solution) and apply it evenly on a filter paper strip (l × w = 6.0 × 0.5 cm), put it into a Pasteur tube, and connect it to a stimulating airflow superior. The flow rate of the stimulating airflow is 1150 mL / min, and the flow rate of the continuous airflow is 850 mL / min. After the baseline was stabilized, artificial stimulation was given with the pedal, and the single stimulation lasted for 0.2 s, with an interval of more than 60 s between each time. The sequence of samples to be tested in the same antennae was n-hexane (1 time), trans-2-hexenal...

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Abstract

The invention discloses applications of 3,4-dimethylbenzaldehyde, particularly applications of the 3,4-dimethylbenzaldehyde in preparation of pest attractants, wherein the 3,4-dimethylbenzaldehyde isone of volatile secondary metabolites synthesized by Chinese rose plants. According to the invention, the active component is separated from the Chinese rose headspace volatile matter, and the results of GC-EAD, EAG, olfactory behavior, oviposition behavior biological tests and field oviposition tests prove that the 3,4-dimethylbenzaldehyde has good attracting effect on pests, especially Helicoverpa armigera female moths; and the 3,4-dimethylbenzaldehyde is a natural secondary metabolite and has a complete degradation path in nature, so that the 3,4-dimethylbenzaldehyde is used as a main component to prepare the pest attractant so as to achieve the pollution-free prevention and control of pests.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to an application of 3,4-dimethylbenzaldehyde. Background technique [0002] Up to now, chemical pesticides are one of the main means to control plant pests. However, chemical pesticides, especially chemical pesticides, will cause environmental pollution, pest resistance, and excessive pesticide residues in agricultural products while eliminating pests. With the increasing requirements of modern agriculture and ecological environment on the performance of chemical pesticide varieties, the risk of research and development of new chemical pesticide varieties is also increasing. However, botanical pesticides have the advantages of diverse biological activities (insecticide, bactericidal, weeding and various modes of action), high selectivity, no residue, safe for the environment, safe for higher animals and natural enemies, and less likely for pests to develop resi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/04A01P19/00
CPCA01N35/04
Inventor 杨发忠周世萍唐敏杨斌李云仙朱国磊陈亮亮
Owner SOUTHWEST FORESTRY UNIVERSITY
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