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A kind of creation method of short style tomato

A tomato and style technology, applied in the field of crop breeding, can solve the problems of inability to pollinate seeds, stigma damage, etc., and achieve the effects of simplifying manual detasseling operations, reducing seed production costs, and improving seed production efficiency

Active Publication Date: 2021-08-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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Problems solved by technology

If the stigma is shortened, you can directly pinch the top of the flower bud with your hands when detasseling, and quickly pull out the petals and anthers without damaging the stigma (the stigma of wild-type tomato is generally flush with or slightly lower than the anther tube, this operation will Damage to the stigma leading to inability to pollinate and set seeds)

Method used

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  • A kind of creation method of short style tomato
  • A kind of creation method of short style tomato
  • A kind of creation method of short style tomato

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Embodiment Construction

[0043] In order to make the object, technical solution and advantages of the present invention clearer, the implementation manner of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0044] 1. Tomato material

[0045] The tomato variety TB0993 used. TB0993 was bred from the hybrid variety group BF001 of Nunhem Company in the Netherlands in 2009 through 8 generations of directional purification system (single plant method to select plants with Ty1 and Ty3a loci resistant to tomato yellow leaf curl virus disease and high fruit setting rate) An excellent inbred line of pink fruit tomato. In the present invention, the whole genome of TB0993 tomato was sequenced, and the sequencing was completed by Beijing Tianyi Huiyuan Biotechnology Co., Ltd., and the sequencing results were not disclosed.

[0046] 2. Gene profile

[0047]According to the literature report of Benoit Gorguet et al. in 2009: PS-2 gene (GenBank number...

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Abstract

The invention discloses a method for creating a short-style tomato. The method uses the CRISPR / Cas9 system to mutate and knock out the 5th or 6th exon of the PS-2 gene of tomato, and the style of the obtained mutant strain is shortened , significantly improved the efficiency of emasculation.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and relates to a method for creating a short-style tomato, in particular to a method for creating tomato short-style germplasm by knocking out the PS-2 gene with a CRISPR / Cas9 system. Background technique [0002] Tomato (Solanum lycopersicum) is a complete flower and a self-pollinated crop, and the tomato seeds sold in production are all hybrid first generation (F1 generation). When producing tomato hybrid seeds, manual detasseling is necessary. During emasculation, the general operation is to separate the flower buds and petals with tools such as tweezers, and then remove the stamens. Due to the small size of tomato flowers, the operation is difficult, time-consuming and labor-intensive. [0003] Improving the efficiency of tomato emasculation can greatly improve the efficiency of tomato hybrid seed production and reduce the cost of seed production. [0004] Shortening the stigma helps...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/56C12N9/24A01H5/02A01H6/82
CPCC12N9/2402C12N15/8213C12Y302/01015
Inventor 周明李常保李传友邓磊李保思
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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