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A method for identifying female ginseng individuals in the ovary development stage of rough sea cucumber

A technology of rough sea cucumber and developmental stage is applied in the field of identifying female ginseng individuals in the ovarian development stage of rough sea cucumber, and can solve the problems of difficulty in selecting parent ginseng of rough sea cucumber and the like.

Active Publication Date: 2021-04-06
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The appearance of female ginseng individuals with developed ovaries is no different from that of gonad-less individuals and male ginseng individuals, which brings difficulties in the selection of parent ginseng during artificial breeding of rough sea cucumbers

Method used

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  • A method for identifying female ginseng individuals in the ovary development stage of rough sea cucumber
  • A method for identifying female ginseng individuals in the ovary development stage of rough sea cucumber
  • A method for identifying female ginseng individuals in the ovary development stage of rough sea cucumber

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Experimental program
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Effect test

Embodiment 1

[0020] 1. SDS-PAGE electrophoresis of coelomic fluid of rough sea cucumber

[0021] (1) Preparation of 100mM protease inhibitor PMSF (phenylmethylsulfonyl fluoride)

[0022] Weigh 174mg of PMSF and dissolve it in 1mL of ethanol to prepare a 174mg / mL storage solution. Then dilute PMSF with a concentration of 174 mg / mL to 100 mM PMSF working solution with 9 mL of PBS.

[0023] (2) Extraction and centrifugation of body cavity fluid of rough sea cucumber

[0024] Select 20 adult rough sea cucumbers with a body length greater than 20cm and a body weight greater than 500g. Use a 2mL syringe pre-added with 20μL of 100mMPMSF to pass through the body wall of rough sea cucumber, insert 2cm to reach the body cavity, and extract the body cavity fluid. The extracted body cavity fluid was centrifuged at 1,000 g in a refrigerated centrifuge at 4°C for 15 min. Discard the precipitate, and transfer the supernatant to a new centrifuge tube for later use.

[0025] (3) Selection of body cavi...

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Abstract

The invention discloses a method for identifying female ginseng individuals in the ovary development stage of rough sea cucumbers. The body cavity fluid of the rough sea cucumber was extracted, and then the yolk protein was separated by centrifugation, and then subjected to SDS-PAGE electrophoresis and staining, and the electrophoresis results were used to distinguish the female ginseng in the ovarian development stage, the male ginseng and the rough sea cucumber with immature gonads. The individual samples of female ginseng at the stage of ovarian development showed a characteristic band of 200‑250kD, while the individual samples of gonadal anaplastic individual and male ginseng did not appear this characteristic band. The invention can identify female ginseng individuals in the ovarian development stage by extracting body cavity fluid samples without damaging the rough sea cucumber, and has application value for the selection of parent ginseng during artificial seedling cultivation.

Description

technical field [0001] The invention belongs to the field of mariculture biotechnology, and in particular relates to a method for identifying female ginseng individuals in the ovary development stage of rough sea cucumbers. Background technique [0002] Coarse sea cucumber (Holothuriascabra), also known as white ginseng and sand ginseng, belongs to the phylum Echinodermata, class Holothuria, subclass Cladochiabra, order Lapis, family Holothuriaceae, and genus Holothuria. Distributed in the west from Port Natal to the Red Sea, east to the Caroline Islands, Fiji Islands, north to Japan, south to Australia, and Guangxi, Guangdong, Hainan and other places in mainland China, generally living on the edge of coastal reefs and Sandy bottom with strong currents and seaweed. Coarse sea cucumber has a thicker body wall, is rich in collagen, and has a smooth and elastic taste. It not only has high nutritional value, but also has extremely high medicinal value. Coarse sea cucumber is a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/30A01K61/90G01N33/68C07K14/775C07K1/14
CPCA01K61/30A01K61/90C07K14/775G01N33/68G01N2333/43504
Inventor 陈廷任春华胡超群李小敏吴菲菲江晓
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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