Aronia melanocarpa tender shoot induction culture method
A kind of technology of Sorbus nigra, cultivating method, applied in horticultural methods, botanical equipment and methods, horticulture, etc., capable of solving problems such as differences in climate conditions
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Embodiment 1
[0034] Step 1: Preparatory work for cultivating hardware: prepare cultivating equipment, prepare culturing materials, prepare culture solutions with different nutritional components, sterilize the culture solutions and place them for later use;
[0035] Step 2: Pretreatment of culture materials: Take the stems and leaves of Aronia nigrutum plants cultivated in the greenhouse, wash the surface of the stems and leaves of the plants with flowing distilled water, rinse continuously for 2 minutes, and then use 75% medical alcohol to clean the surface of the plants. Sterilization treatment, after the outer surface of the plant has been fully treated;
[0036] Step 3: Preliminary operation of cultivation: Soak in 0.1-0.2% mercuric chloride solution for 10 minutes, then rinse the stems and leaves of the plant with flowing distilled water several times, and finally take out the surface tender leaves, and cut the pedicel into 2-3mm with scissors Thin slices, placed flat on the surface o...
Embodiment 2
[0044]Step 1: Preparatory work for cultivating hardware: prepare cultivating equipment, prepare culturing materials, prepare culture solutions with different nutritional components, sterilize the culture solutions and place them for later use;
[0045] Step 2: Pretreatment of culture materials: Take the stems and leaves of Aronia nigrutum plants cultivated in the greenhouse, wash the surface of the stems and leaves of the plants with flowing distilled water, rinse continuously for 2 minutes, and then use 75% medical alcohol to clean the surface of the plants. Sterilization treatment, after the outer surface of the plant has been fully treated;
[0046] Step 3: Preliminary operation of cultivation: Soak in 0.1-0.2% mercuric chloride solution for 12 minutes, then rinse the stems and leaves of the plant with flowing distilled water several times, and finally take out the surface tender leaves, and cut the pedicels into 2-3mm diameters with scissors Thin slices, placed flat on the...
Embodiment 3
[0054] Step 1: Preparatory work for cultivating hardware: prepare cultivating equipment, prepare culturing materials, prepare culture solutions with different nutritional components, sterilize the culture solutions and place them for later use;
[0055] Step 2: Pretreatment of culture materials: Take the stems and leaves of Aronia nigrutum plants cultivated in the greenhouse, wash the surface of the stems and leaves of the plants with flowing distilled water, rinse continuously for 2 minutes, and then use 75% medical alcohol to clean the surface of the plants. Sterilization treatment, after the outer surface of the plant has been fully treated;
[0056] Step 3: Preliminary operation of cultivation: Soak in 0.1-0.2% mercuric chloride solution for 14 minutes, then rinse the stems and leaves of the plant with flowing distilled water several times, and finally take out the surface tender leaves, and use scissors to cut the pedicel into 2-3mm Thin slices, placed flat on the surface...
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