Method of constructing electrochemical immunosensing interface based on conductive gel with active sites

Abstract

The invention relates to a method of constructing an electrochemical immunosensing interface based on conductive gel with active sites and belongs to the field of immunoassay or electrochemical sensing. According to the purposes of the method, firstly, the host-guest recognition action of vinyl ferrocene and monoaldehyde cyclodextrin is utilized to obtain a supramolecular complex, then the shape-controlled conductive gel is synthesized through copolymerization by use of a mixed solvent method and is loaded to a working electrode, and finally the electrochemical immunosensing interface is obtained through an aldehyde-amine condensation reaction of chemically immobilized antibodies. According to the method, preparation conditions are mild, the reaction is rapid, the obtained interface has excellent conductivity, meanwhile a large quantity of antibodies can be fixed, finally sensitivity recognition can be directly performed on to-be-detected antigens through current signal changes, and therefore the electrochemical immunosensing interface has broad application prospects in immunoassay and electrochemical sensing fields.

Description

technical field [0001] The invention relates to a method for constructing an electrochemical immunosensing interface by using a conductive gel with an active site, and belongs to the field of immunodetection or electrochemical sensing. Background technique [0002] Electrochemical immunosensing is a combination of electrochemical analysis technology and immunodetection, and realizes the analysis of the analyte by using the specific recognition of antigens and antibodies and the change of electrical signals in electrochemistry. With its strong specificity and high sensitivity , good accuracy and easy operation, electrochemical immunosensing has shown great development prospects in the detection of cancer, heart disease and various metabolic diseases. How to improve its detection effect has always been one of the hot spots that researchers are most concerned about. Among them, since the recognition of the antibody and the antigen to be tested and the electron transfer between...

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Problems solved by technology

[0004] However, conductive gels are usually inert, and it is very difficult to introduce binding sites on their molecular backbones.

Although copolymerization of conductive monomers with active site-containing units such as acrylic acid can easily introduce binding sites, non-conductive active units will destroy the continuous Π-conjugated network structure in the gel, hinder electron transport, and seriously weaken its conductivity.

Therefore, how to prepare a conductive gel electrochemical sensing interface with active sites and maintain excellent conductivity still faces great challenges.

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