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Method for rapidly predicting heterosis based on genome size of crops

A technology of heterosis and genome, applied in the direction of plant genetic improvement, botanical equipment and methods, applications, etc., can solve the problems of inability to reproduce through grafting, achieve shortening the breeding cycle, strong operability, and reduce breeding costs Effect

Pending Publication Date: 2020-01-17
CHENGDU ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

This technical solution realizes the prediction of heterosis for different varieties by grafting the parental values, affinity values, and interaction values ​​of the two varieties, and calculates the target yield and related physiological indicators of different grafting types, which is beneficial to Improves the efficiency of cross-breeding, but this method requires multiple types of grafting and cultivation management of the parents, and is not suitable for crops that cannot be reproduced by grafting

Method used

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  • Method for rapidly predicting heterosis based on genome size of crops
  • Method for rapidly predicting heterosis based on genome size of crops
  • Method for rapidly predicting heterosis based on genome size of crops

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Embodiment 1

[0049] Such as figure 2 As shown, taking Brassica napus as an example, this embodiment provides a method for quickly predicting heterosis based on genome size, including the following steps:

[0050] S1. Using 12 genetically stable tetraploid Brassica napus as parent crops, measure the genome size of these 12 materials by flow cytometry, and calculate the relative difference percentage of genome size between any pair of materials in the 12 materials In the range of 0.7 to 18.9%. The Nei genetic distance between pairs of materials was found to be in the range of 0.33-0.74 by SNP chip typing. Among them, there are 48 hybrid combinations that meet the conditions of a. The relative difference percentage of the genome size of the two parent crops is 2-20% and b. The Nei genetic distance of the two parent crops is greater than or equal to 0.35, and then combine 12 materials traits (growth period, resistance, yield, quality and other traits), a total of 23 groups of hybrid combina...

Embodiment 2

[0059] Such as Figure 5 As shown, taking rice as an example, this embodiment provides a method for rapidly predicting heterosis based on genome size, including the following steps:

[0060] S1. 16 genetically stable diploid indica varieties were used as parent crops. The genome size of these 16 materials was determined by flow cytometry. The relative difference percentage of genome size between pairs of materials in 16 materials was calculated in the range of 0.3-15.2%, and the Nei genetic distance between pairs of materials was in the range of 0.24-0.67 through rice 50K SNP microarray typing. Among them, there are 53 hybrid combinations that meet the conditions a. The relative difference percentage of the genome size of the two parent crops is 2-20% and b. The Nei genetic distance of the two parent crops is greater than or equal to 0.35, and then 16 materials are combined. Traits (growth period, resistance, yield, quality and other traits), screen out consistent growth per...

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Abstract

The invention discloses a method for rapidly predicting heterosis based on genome size, and belongs to the technical field of crop breeding. The method includes the following steps: S1. selecting a hybrid combination parent according to the relative difference percentage of genome size and Nei genetic distance between crops; S2. emasculating and hybridizing the selected hybrid combination parent to obtain F1 generation; S3. carrying out heterosis analysis on target traits of the F1 generation; S4. analyzing the correlation between heterosis of the target traits of the F1 generation and Nei genetic distance between parents; S5. analyzing the correlation between heterosis of the target traits of the F1 generation and absolute value of genome size difference between parents; and S6. combiningthe above analysis results, selecting strong heterosis hybrid combinations and predicting heterosis of the F1 generation at the same time. The breeding method is beneficial to improving breeding efficiency and reducing breeding cost, and has the advantage of wide applicability.

Description

technical field [0001] The invention belongs to the technical field of crop breeding and relates to a method for quickly predicting heterosis based on the size of the genome of crops. Background technique [0002] Heterosis refers to the phenomenon that the hybrids produced by the hybridization of two parents with different traits (or genetic composition) surpass their parents in terms of vigor, growth, resistance and high yield. It is a common biological law. At present, using heterosis for hybrid breeding is one of the main ways to improve the yield, resistance and quality of crops. However, the genetic traits of the hybrid F1 generation produced after the crossing of parents (male parent and female parent) are uncertain, not all hybrid offspring have hybrid vigor, and the heterosis of offspring produced by different parent combinations also varies widely. Some of them had obvious heterosis, some were not obvious, and even degenerated. [0003] In the practical applicati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 付绍红朱振东李云杨进王继胜邹琼康泽明陶兰蓉唐蓉
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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