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Streptomyces 5017 and its application in antagonizing plant pathogens

A technology of plant pathogenic bacteria, Streptomyces, applied in the field of microbiology

Active Publication Date: 2022-07-29
GUANGXI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are many studies on the use of Streptomyces from terrestrial sources in the field of biocontrol, while there are relatively few studies on the use of Streptomyces from marine sources as biocontrol agents to antagonize plant pathogens, especially banana wilt

Method used

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  • Streptomyces 5017 and its application in antagonizing plant pathogens
  • Streptomyces 5017 and its application in antagonizing plant pathogens
  • Streptomyces 5017 and its application in antagonizing plant pathogens

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Embodiment Construction

[0031] 1 Materials and methods

[0032] 1.1 Materials to be tested

[0033] 1.1.1 Isolation and purification of strains

[0034] Collect the rhizosphere soil of mangrove plants in Beibu Gulf, Guangxi (within 5mm from the roots), weigh 2.0g soil samples and put them in a conical flask of 20ml sterile water (with glass beads), shake them up by hand; make 10 in turn; -2 and 0 -3 Diluted sample solution. 200 μl of samples were taken and placed in 6 different culture media for coating, and cultured in a 28°C incubator for 20 days. The morphological characteristics of the colonies were observed, and the colonies were picked for purification. The purified strains were placed in 20% (v / v) glycerol and frozen at -80°C.

[0035] The tested plant pathogenic bacteria strains Banana anthracis, mango anthracnose, Plumeria pearl anthracis, Botrytis, Fusarium oxysporum No. 4 and No. 1, and Alternaria were all provided by the Guangxi Academy of Agricultural Sciences.

[0036] 1.1.2 Test m...

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Abstract

The invention discloses a strain of Streptomyces sp. B5017, which is characterized in that the preservation number is CCTCC NO: M2019648 and the classification name is Streptomyces sp. B5017. Tests show that the new marine-derived Streptomyces strain of the present invention has the effect of antagonizing various plant pathogenic bacteria, and is effective against banana anthracnose, mango anthracnose, pearl anthracnose, Phytophthora vinifera, and Fusarium oxysporum No. 4 race. , Banana Fusarium oxysporum No. 1 race or Alternaria have inhibitory effect. The fermented liquid prepared by Streptomyces 5017 of the present invention can effectively avoid the yellowing and wilting of leaves caused by Fusarium oxysporum No. 4 and No. 1 races in pot experiments, which proves that the Streptomyces 5017 can effectively prevent and treat banana fusarium wilt, It has good ecological and social benefits to enhance the disease resistance of bananas, and has a good application prospect as a biocontrol preparation.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Streptomyces 5017 and its application in antagonizing plant pathogenic bacteria. Background technique [0002] As the world's second largest fruit crop, bananas are rich in potassium, magnesium and crude fiber, rich in nutrients, and also the largest fruit in world trade, with high economic value. Banana fusarium wilt caused by Fusarium oxysporumf.sp.cubense.Foc limits the cultivation and sustainable development of bananas due to its rapid spread, rapid onset and serious damage. At present, there are no effective control methods and chemical agents that can completely control the disease. The long-term use of chemical fungicides can easily damage the ecological environment of the soil, promote the resistance of pathogenic bacteria, and endanger human health. [0003] In recent years, the application of microorganisms to control banana fusarium wilt has been...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCA01N63/00C12R2001/465C12N1/205
Inventor 胡文进潘信利李菲黄媛林李其利黄庶识陈东李喆陆璐
Owner GUANGXI ACAD OF SCI
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