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Quercus mongolica Fisch.ex Ledeb SSR molecular marker and corresponding primer and application thereof

A technology of molecular markers and reaction procedures, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as low genetic gain of improved varieties, inability to meet improved varieties, insufficient supply capacity, etc., and achieve results Stable and reliable, easy to detect, fast response effect

Active Publication Date: 2019-12-20
LIAONING FORESTRY SCI RES INST
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Problems solved by technology

At present, the research on the breeding of Quercus mongolica improved varieties is mainly based on tree growth indicators, seed growth indicators and physiological indicators, and most of them are provenance selection. The improved varieties selected by these methods have low genetic gain, low yield and insufficient supply capacity. Unable to meet production demand for improved varieties

Method used

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  • Quercus mongolica Fisch.ex Ledeb SSR molecular marker and corresponding primer and application thereof
  • Quercus mongolica Fisch.ex Ledeb SSR molecular marker and corresponding primer and application thereof
  • Quercus mongolica Fisch.ex Ledeb SSR molecular marker and corresponding primer and application thereof

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Embodiment 1

[0111] In the sequence listing of the present invention, SEQ ID NO: 1 to SEQ ID NO: 10 are the nucleotide sequences of Quercus mongolica genome SSR sites N1010265, N1014482, N1105306, N1110207, N1345390, N12361, N1247890, N1457047, N4544558, N4529840.

[0112] In the sequence listing of the present invention, SEQ ID NO: 11 to SEQ ID NO: 30 are primers corresponding to the SSR sites of Quercus mongolica genome (N1010265-F, N1010265-R, N1014482-F, N1014482-R, N1105306-F, N1105306- R, N1110207-F, N1110207-R, N1345390-F, N1345390-R, N12361-F, N12361-R, N1247890-F, N1247890-R, N1457047-F, N1457047-R, N4544558-F, N-4544558 N4529840-F, N4529840-R) nucleotide sequences.

[0113] 1. Quercus mongolica genome SSR locus and primer development

[0114] Microsatellite DNA refers to the DNA tandem repeat sequence composed of short repeating units (generally 1 to 6 bases) in the genome, also known as short tandem repeats (Short Tandem Repeats, STRs) or simple repeats (Simple Sequence Repeat...

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Abstract

The invention belongs to the field of a molecular biology DNA labeling technique, and particularly relates to a quercus mongolica Fisch.ex Ledeb SSR molecular marker and a corresponding primer and application thereof. The nucleotide sequence of 10 SSR sites is SEQID NO: 1 to SEQID NO: 10 sequences in a sequence table, and the nucleotide sequence of the corresponding primer is SEQID NO: 11 to SEQIDNO: 30 sequences in the sequence table. According to the quercus mongolica Fisch.ex Ledeb SSR molecular marker and the corresponding primer and application thereof, a technical support can be provided for identification of quercus mongolica Fisch.ex Ledeb, protection of germplasm sources, and breeding of fine varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular biology DNA markers, and in particular relates to a Quercus mongolica SSR molecular marker, corresponding primers and applications thereof. Background technique [0002] Mongolian Quercus (Quercus mongolica Fisch.ex Ledeb) belongs to Fagaceae, Quercus, a deciduous tree, up to 30 meters, with grayish-brown bark and deep longitudinal fissures. Crown oval. Alternate single leaves, obovate leaves, wavy tooth edges, dark green leaf surface, light green backside, unisexual flowers, monoecious plants, ovate or oval nuts, fleshy cotyledon seeds, is a national second-class precious tree species in China, and The main secondary forest tree species in the forest regions of Northeast China. It is mainly distributed in Northeast China, North China, and Northwest China, and a small amount is also distributed in Central China. It is also distributed in Russia, Japan, Mongolia and the Korean Peninsula. Quer...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 陈罡冯健于世河王骞春陆爱君卜鹏图王占伟郑颖张砚辉王敬贤刘怡菲
Owner LIAONING FORESTRY SCI RES INST
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