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A kind of indel molecular marker closely linked with pepper cluster inflorescence gene, primer and application

A molecular marker, pepper technology, applied in the field of pepper breeding and molecular biology, can solve problems such as the unresolved plant type morphological structure regulation mechanism, weak genetic basis research, and few pepper plant types, so as to reduce the planting scale and later stages. Identify workload, improve efficiency and accuracy

Active Publication Date: 2022-06-07
HUNAN AGRICULTURAL UNIV +1
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Problems solved by technology

[0004] Although the important value of plant structure to crop production is well known, the genetic basis of its growth habit variation is relatively weak. There are relatively few studies on pepper plant structure, and the regulation mechanism of plant structure has not yet been effective. answer

Method used

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  • A kind of indel molecular marker closely linked with pepper cluster inflorescence gene, primer and application
  • A kind of indel molecular marker closely linked with pepper cluster inflorescence gene, primer and application
  • A kind of indel molecular marker closely linked with pepper cluster inflorescence gene, primer and application

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Experimental program
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Effect test

Embodiment 1

[0036] A pepper cluster molecular marker InDel-9258, obtained by the following method:

[0037] 1. Group building

[0038] Using pepper LS20 single inflorescence inbred line as the female parent, XJ10 pepper cluster inflorescence inbred line as the male parent, F1 was obtained by crossing, and F1 was selfed to obtain F2, and the F2 population was constructed ( figure 1 ).

[0039] 2. Inflorescence phenotype identification

[0040] (1) Parental LS20 and XJ10, F1 and F2 populations were sown in plug trays, and after seedlings were established, they were transplanted into glass greenhouses for routine cultivation and management.

[0041] (2) Phenotypic identification of the F2 population was carried out at the full flowering stage, and 30 F2 individual plants of single and clustered inflorescences were selected to construct two mixed pools.

[0042] 3. Clustered inflorescence QTL analysis

[0043] The RNAs of individual flower buds in selected mixed pools were extracted, and ...

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Abstract

The invention discloses an Indel molecular marker closely linked with pepper cluster inflorescence gene, primer and application. Two highly homozygous inbred lines were used to construct the F2 population. Chromosomal regions closely linked to pepper cluster inflorescences were obtained by BSR and linkage mapping methods, and insertion / deletion locus (InDel) markers were developed in candidate intervals for verification. Finally, the InDel closely linked with the candidate gene was obtained, and the molecular marker In-9258 was designed according to the marker, and the genotype identification of 100 randomly sampled individuals from the F2 population was carried out with the marker, and the coincidence rate reached 100%. The research results can not only be used in the identification of pepper inflorescence types and molecular assisted breeding, but also provide a basis for the map-based cloning of pepper cluster inflorescence genes and the analysis of the molecular mechanism of cluster inflorescence formation.

Description

technical field [0001] The invention belongs to the field of pepper breeding and molecular biology, more particularly relates to an Indel molecular marker and primers closely related to pepper cluster inflorescence, and also relates to their application in predicting pepper inflorescence and molecular assisted breeding. Background technique [0002] Pepper (Capsicum annuum L.) is a vegetable crop of the Solanaceae capsicum genus, native to Central and South America. Because of its rich nutritional value and strong adaptability, pepper is widely grown as an important vegetable crop all over the world, and it is very popular among consumers. According to the statistics of FAO in 2014, the global output of pepper has exceeded 49 million tons. According to the statistics of the bulk vegetable system of the Ministry of Agriculture of my country, the pepper cultivation area in China has been increasing, and the pepper industry has made great progress. In recent years, the annual p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6895C12Q1/6806C12Q2600/13C12Q2600/156
Inventor 欧立军吕俊恒刘周斌刘宇华陈文超邹学校于会洋陈蓉欧阳波李峰
Owner HUNAN AGRICULTURAL UNIV
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