Early CRC (Colorectal Cancer) diagnostic marker circ4953 and application thereof

A technology for diagnosing markers and colorectal cancer, applied in the field of tumor markers, can solve the problems of early diagnosis and insufficient treatment of CRC, and achieve the effect of reducing the degree of trauma and diagnosis cost, with excellent sensitivity and specificity

Active Publication Date: 2019-12-20
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that early diagnosis of CRC can significantly improve the 5-year survival rate of patients, but the lack of early specific markers leads to a serious shortage of early diagnosis and treatment of CRC

Method used

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  • Early CRC (Colorectal Cancer) diagnostic marker circ4953 and application thereof
  • Early CRC (Colorectal Cancer) diagnostic marker circ4953 and application thereof
  • Early CRC (Colorectal Cancer) diagnostic marker circ4953 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1, high-throughput sequencing screening of circRNAs associated with early colorectal cancer

[0021] This study is based on high-throughput sequencing, and the specific screening process is as follows:

[0022] 1. Sample collection:

[0023] The inventor collected 28 cases of early CRC tissues and 28 cases of normal tissues adjacent to the tumor (5cm away from the cancer tissue) from the Department of Colorectal Surgery, the First Affiliated Hospital of Zhengzhou University (the samples used for research were sampled, subpackaged, and stored under uniform conditions) , The patient did not receive any preoperative anti-cancer treatment when the sample was taken. After the tissue was separated from the human body, the tissue was washed with normal saline to remove blood stains, and then quickly transferred to liquid nitrogen for storage. During the experiment, repeated freezing and thawing of tissues was avoided as much as possible, and the experimental data rest...

Embodiment 2

[0033] Example 2, verifying differential expression

[0034] 1. Sample collection

[0035] On the basis of Example 1, using the same treatment method, the inventor expanded the volume from 28 pairs of tissues to 39 pairs, a total of 39 cases of early CRC tissues and 39 cases of normal tissues adjacent to the tumor. In addition, the inventor collected serum samples from 19 early-stage CRC patients and 19 healthy controls, collected 2-3ml of whole blood in vacuum blood collection tubes with red caps (without anticoagulants and coagulants), and left them at room temperature for 1 hour , centrifuged at 3000rpm for 10min, collected supernatant 1ml, and stored at -80°C for later use; the above research was approved by the ethics committee, and the patient gave informed consent.

[0036] 2. Trizol method for RNA extraction

[0037] Total RNA was isolated from serum and early CRC tissues using RNAiso Plus (TaKaRa) according to the manufacturer's instructions.

[0038] 2.1 After the...

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Abstract

The invention provides an early CRC (Colorectal Cancer) diagnostic marker. The diagnostic marker is circular RNA circ4953, and has the nucleotide sequence shown as SEQ ID No.1. During the applicationof the circular RNA circ4953 to preparation of an early CRC diagnosis tool, the tool is a kit, and comprises a primer pair for amplifying and specifically recognizing the nucleotide sequence of the circular RNA circ4953. The occurrence of the early colorectal tumor diseases can be explicitly and clearly expressed; the expression of the marker in a plurality of early CRC tissue samples is obviouslyhigher than the expression in a control tissue sample (normal tissues with a distance being 5cm from tumor tissues); and the expression of the marker in a plurality of early CRC patient blood serum samples is obviously higher than the expression in control healthy blood serum samples.

Description

technical field [0001] The present invention relates to the technical field of tumor markers, in particular to an early colorectal cancer diagnostic marker circ4953 and its application. Background technique [0002] Colorectal cancer (CRC) is a high-incidence tumor of the digestive system, which has a high degree of malignancy and seriously affects the survival of patients. Studies have shown that early diagnosis of CRC can significantly improve the 5-year survival rate of patients, but the lack of early specific markers leads to a serious shortage of early diagnosis and treatment of CRC. Therefore, it is urgent and necessary to find diagnostic markers for early CRC. [0003] Circular RNA (circRNA) is a special type of non-coding RNA that plays an important regulatory role in disease. As tumor biomarkers, circRNAs have many advantages. CircRNA has a closed and circular structure, is not easily degraded by exonucleases, and is more stable than linear RNA. In addition, cir...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/178C12Q2600/158Y02A50/30
Inventor 孙振强郭雅欣汲翔袁维堂汲振余陈晨范丹丹刘金波赵陆洋邵博
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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