Separation method of mesenchymal stem cells

A technology of mesenchymal stem cells and separation methods, applied in the field of mesenchymal stem cell separation, can solve the problems of not being suitable for large-scale cultivation of mesenchymal stem cells, and achieve the effects of easy separation, high operating efficiency, and improved separation yield

Inactive Publication Date: 2019-12-20
广州乾晖生物科技有限公司
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Problems solved by technology

[0003] In the prior art, there are many methods for the isolation, cultivation and purification of mesenchymal stem cells. However, due to various defects in the existing methods, there is no method suitable for large-scale cultivation of clinically applicable mesenchymal stem cells.

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  • Separation method of mesenchymal stem cells
  • Separation method of mesenchymal stem cells
  • Separation method of mesenchymal stem cells

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Embodiment Construction

[0041] The embodiments described below are exemplary, and are only used to explain the present application, and cannot be construed as limiting the present application. Also, detailed descriptions of known technologies will be omitted if they are unnecessary to illustrate the features of the present application.

[0042] In the example of this application, with the approval of the Ethics Committee of the Medical Center of a tertiary hospital in Guangzhou and the written informed consent of the parturient and family members, the obstetrics and gynecology department of a tertiary hospital in Guangzhou took patients who were under 35 years old and negative for infectious diseases. Placenta of women with a gestational age of more than 35 weeks, rupture of membranes not exceeding 18 hours, regular check-ups, no abnormalities, no infection or other diseases that may interfere with placental vitality (retain 25-35cm umbilical cord), as human placenta organize.

[0043] see figure 1...

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Abstract

The invention provides a separation method of mesenchymal stem cells. The separation method comprises the following steps: complete human placental tissue is taken, and cleaning perfusion liquid is poured from an umbilical vein of the placental tissue till the cleaning perfusion liquid flowing out of the placental tissue is colorless; the placental tissue is sealed to enable an impermeable perfusion space to be formed in the placental tissue, and enzyme perfusion liquid is poured from the umbilical vein of the placental tissue for digestion; first target cells containing the mesenchymal stem cells in the enzyme perfusion liquid are collected and cultured; and the first target cells are cultured to the logarithmic growth phase, and 1 / 3 of free cells of the first target cells are retained tobe subjected to subculture so as to amplify the mesenchymal stem cells. The separation yield of the mesenchymal stem cells can be greatly increased.

Description

technical field [0001] The present application relates to the field of stem cells, in particular, the present application relates to a method for isolating mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells exist in a variety of tissues and have multiple differentiation potentials such as adipogenic, osteogenic, chondrogenic, adipogenic, neurogenic, bone marrow stromal and even liver cells. They are a kind of stem cells in cell therapy and tissue engineering research Important seed cells and gene therapy vectors. Its clinical transformation application has become a research hotspot, among which how to obtain a large number of primary mesenchymal stem cells is the key to various researches. [0003] In the prior art, there are many methods for the isolation, cultivation and purification of mesenchymal stem cells. However, due to various defects in the existing methods, there is no method suitable for large-scale cultivation of clinically applicable ...

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00
Inventor 高毅易笑陈枫李阳
Owner 广州乾晖生物科技有限公司
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