N-terminal B-type brain natriuretic peptide precursor magnetic particle chemiluminiscence detection kit

Abstract

The invention belongs to the field of biotechnology detection and relates to an N-terminal B-type brain natriuretic peptide precursor magnetic particle chemiluminiscence detection kit. The kit comprises an NT-proBNP antibody-coated magnetic microsphere suspension, an NT-proBNP enzyme conjugate, an NT-proBNP reference substance, an NT-proBNP quality control substance, an NT-proBNP concentrated washing solution, a substrate solution A and a substrate solution B. In practical application, the kit has the advantages of high sensitivity, high accuracy, low intra-batch and inter-batch difference, wide linear range, and greatly improved e detection performance.

Description

technical field [0001] The invention relates to the field of biotechnology detection, in particular to an N-terminal B-type brain natriuretic peptide precursor magnetic particle chemiluminescent detection kit. Background technique [0002] As a quantitative marker of heart failure, N-terminal pro-B-type natriuretic peptide (NT-proBNP) not only reflects left ventricular systolic dysfunction, but also reflects left ventricular diastolic dysfunction, valve dysfunction and right ventricular dysfunction. NT-proBNP is a quantitative marker of heart failure (Heart Failure, referred to as HF). NT-proBNP has a high degree of accuracy in diagnosing HF and can be used to guide the curative effect of treating chronic HF. NT-proBNP is also a predictor of death in patients with acute coronary syndrome. [0003] In the current existing technology, the N-terminal B-type BNP precursor chemiluminescence detection kit is mainly plate or tube chemiluminescence, which can only realize the detec...

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Problems solved by technology

[0003] In the current existing technology, the N-terminal B-type BNP precursor chemiluminescence detection kit is mainly plate or tube chemiluminescence, which can only realize the detection result within 65 minutes; the analytical sensitivity is not higher than 15pg / mL; The intra-assay precision is not higher than 15%, and the inter-assay precision is not more than 20%. There are shortcomings such as low test sensitivity and accuracy, high intra-assay and inter-assay differences, and narrow linear range, which are increasingly unsatisfactory. Higher-level requirements for modern medical testing

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