Method for investigating relationship between lung cancer clinical pathology and gene expression

Abstract

The invention belongs to the field of medical exploration methods, and provides a method for investigating the relationship between lung cancer clinical pathology and gene expression; the method is used for guiding personalized and accurate lung cancer treatment and prognosis in the aspect of gene mutation. The method comprises the steps: confirmation of candidate genes; selection of a target gene; establishment of database; verification of lung cancer candidate genes; relevance of the target gene with lung cancer prognosis and clinical pathology characteristics; verification of expression ofthe target gene in lung adenocarcinoma; screening of a target gene low-expression lung cancer cell strain; influence of target gene overexpression / interference on lung cancer cell in-vitro invasion and metastasis; hypothesis of regulation and control of the target gene on the aspects of cell cycle apoptosis and invasion; regulation and control experiments of the target gene on the aspects of cellcycle apoptosis and invasion; and influence of target gene overexpression on a downstream signal path. In combination with various databases, various hypotheses are provided, the correctness of the hypotheses is verified through the experiments, and the next diffusion condition of lung cancer cells and the information interaction condition between cancer cells are predicted.

Description

technical field [0001] The invention belongs to the field of medical exploration methods, in particular to a method for investigating the relationship between clinical pathology and gene expression of lung cancer. Background technique [0002] Lung cancer is the most common malignant tumor in the world. Worldwide, lung cancer occupies the first place in the cause of cancer death in men, and it is also the second most common cause of cancer death in women (after breast cancer). The incidence of lung cancer in my country shows an increasing trend year by year, with an average annual increase of nearly 2%. Lung cancer is divided into multiple tissue types according to different pathological characteristics, and the treatment measures are also different for different lung cancer tissue types. According to the 2004 edition of the WHO classification, common histopathological types of lung cancer are divided into non-small cell carcinoma (NSCLC) and small cell carcinoma (SCLC). N...

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Problems solved by technology

[0007] Currently, 30%-50% of lung adenocarcinomas in general have no known driver alteration genes, and these patients cannot benefit from targeted therapy