A quality inspection method for Yemachai

A quality inspection method and technology of Mustang, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as the inability to comprehensively and objectively evaluate the quality of Mustang Chase.

Active Publication Date: 2020-07-24
南京市中西医结合医院
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Problems solved by technology

[0003] Yemazhui contains active ingredients such as flavonoids, alkaloids, terpenoids, organic acids, coumarins, and volatile oils; the current quality detection method for Yemazhui only uses conventional HPLC to measure 3 to 5 ingredients contained in it. In addition, the current There are reports on the detection of volatile oil components of Yemazhui by GC-MS, but it still fails to fully detect the content of its active ingredients, and cannot comprehensively and objectively evaluate the quality of Yemazhui

Method used

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  • A quality inspection method for Yemachai
  • A quality inspection method for Yemachai
  • A quality inspection method for Yemachai

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of quality detection method of wild horse chasing, it is characterized in that, comprises the following steps:

[0050] (1) Preparation of test solution

[0051] Accurately weigh 20g of the above-mentioned 10 batches of Yemachai medicinal materials, add 100ml of ethanol to extract, use continuous reflux extraction method to extract twice, each time for 1 hour, combine the extracts, filter, and take the subsequent filtrate to pass through a 0.22μm filter membrane to obtain the test product solution.

[0052] (2) Preparation of mixed reference solution

[0053] Accurately weighed Yemai lactone A, Yemai lactone B, Yemai lactone F, Yemai lactone K, Yemai lactone H, rutin, quercetin, kaempferol, hyperoside, iso Rhamnetin, luteolin, dandelion sterol acetate, pseudodandelion sterol acetate, pseudodandelion sterol, scopoletin, 6,7-dimethoxycoumarin, chlorogenic acid, neochlorogenic acid, The reference substances of cryptochlorogenic acid, Eupatorium lutein and Eupator...

Embodiment 2

[0067] Embodiment 2 methodological investigation

[0068] 1. Precision test

[0069] Get above embodiment 1 mixed reference substance solution, according to the chromatographic and mass spectrometry conditions of embodiment 1, continuous sample injection 6 needles respectively on the first day and second day, measure 21 kinds of chemical composition peak areas, and calculate its relative standard deviation (RSD %), the results are shown in Table 4.

[0070] 2. Stability test

[0071] Get about 1.0g of Yemachai sample powder (s1), prepare need testing solution according to the method of Example 1, according to the chromatographic and mass spectrometry conditions of Example 1, sample introduction and determination of 21 The peak area of ​​each chemical component was calculated and its relative standard deviation (RSD%) was calculated. The results are shown in Table 4.

[0072] 3. Repeatability test

[0073] Get about 1.0g of Yemachai sample powder (S1), weigh 6 parts in par...

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Abstract

The invention discloses a quality detection method of lindley eupatorium herb. The method comprises the steps of preparation of a test solution, preparation of a mixed reference substance, establishment of a linear regression equation and content determination. According to the detection method, 21 different active compounds including flavonoid, terpene lactone, phenolic acid, alkaloid and coumarin in the lindley eupatorium herb can be simultaneously detected by screening through a large number of experiments and adopting ultra-high performance liquid chromatography detection. The quality detection method is high in detection sensitivity and good in stability, can objectively, comprehensively and accurately evaluate the quality of the lindley eupatorium herb, and has important significancein controlling the quality and ensuring the curative effect.

Description

technical field [0001] The invention relates to a quality detection method of Chinese medicinal materials, in particular to a quality detection method of Ye Ma Zhui. Background technique [0002] Wild horse chasing is the dry aboveground part of Eupatorium lindleyanum DC. of Compositae, also known as white drum nails, herbivores, and Mao Zelan. It has the effects of clearing away heat and detoxification, eliminating phlegm and relieving asthma, lowering blood pressure, diuresis and reducing swelling. It is mainly used to treat chronic bronchitis, bronchitis, and hypertension. Yemazhui syrup made from it is widely used clinically. , chronic tracheitis, bronchitis has a very good effect. [0003] Yemazhui contains active ingredients such as flavonoids, alkaloids, terpenoids, organic acids, coumarins, and volatile oils; the current quality detection method for Yemazhui only uses conventional HPLC to measure 3 to 5 ingredients contained in it. In addition, the current There ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72
Inventor 王超
Owner 南京市中西医结合医院
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