Molecularly imprinted fluorescent fiber optic sensor and its construction method, fluorescence detection method

Abstract

The invention discloses a molecularly imprinted fluorescent optical fiber sensor, its construction method, and a fluorescent detection method. The sensor includes a laser light source, a Y-shaped optical fiber, a quartz optical fiber, a flange adapter, and an optical fiber spectrometer, and the laser light source, a Y-shaped optical fiber, and an optical fiber spectrometer connected in sequence, the silica fiber modifies the gel film containing molecularly imprinted microspheres, and the Y-shaped fiber is connected to the silica fiber through a flange adapter; the laser light emitted by the laser light source is transmitted to the gel film on the silica fiber Adhesive film, the fluorescent signal of the gel film is reflected back to the Y-shaped optical fiber, and then transmitted to the fiber optic spectrometer by the Y-shaped optical fiber. The sensor of the present invention is a detachable sensor, so that the optical fiber probe can be replaced, so as to perform rapid detection of multiple targets on the spot, can be applied to the on-site rapid monitoring of antibiotics in environmental water, and the optical fiber probe can be easily destroyed after use The modified sensing film and the fiber optic probe can be recycled to reduce unnecessary waste of resources.

Description

technical field [0001] The invention relates to a molecularly imprinted fluorescent optical fiber sensor, a construction method thereof, and a fluorescent detection method, and belongs to the field of antibiotic detection in water environment. Background technique [0002] In recent years, with the rapid development of light technology, various fiber-optic sensors (FOS) have emerged as the times require. This is because FOS has the following advantages: First, FOS has the characteristics of miniaturization. Its sensing part is flexible, light, and has good space adaptability and biocompatibility. It is suitable for real-time and online monitoring of clinical medicine, living organisms and various environmental water bodies. Secondly, FOS has the advantages of wide monitoring objects and great flexibility. In particular, the development of sensing membrane technologies such as molecularly imprinted membranes, polymer membranes, and Sol-gel membranes has provided more feasibl...

AI Technical Summary

Problems solved by technology

[0005] The first purpose of the present invention is to provide a molecularly imprinted fluorescent optical fiber sensor to solve the problems that most of the existing optical fiber sensors cannot be replaced, cannot perform on-site multi-target monitoring, and cannot be eluted and recovered after the optical fiber probe is modified with the sensing film , the sensor is a detachable sensor, so that the fiber optic probe can be replaced, so as to quickly detect multiple targets on the spot, it can be applied to the rapid on-site monitoring of antibiotics in environmental water, and the fiber optic probe can be easily destroyed after use. Sensing film, the fiber optic probe can be recycled, reducing unnecessary waste of resources

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