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A kind of extraction method of berberine in Cortex Phellodendri

A technology of medium berberine and extraction method, which is applied in the field of extraction of berberine from Phellodendron cortex, can solve the problems of difficult filtration and high consistency of water extract, and achieve the effects of increasing extraction rate, increasing solubility, and increasing permeability

Active Publication Date: 2020-08-25
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of extracting method of berberine in Cortex Phellodendri, which carries out pretreatment to Cortex Phellodendri through microbial fermentation, can effectively solve the consistency of the water extract due to the swelling of mucilage to form mucilage in the traditional water extraction process of berberine in Cortex Cortex High and difficult to filter problems, improve the extraction rate of berberine

Method used

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  • A kind of extraction method of berberine in Cortex Phellodendri

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Experimental program
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Effect test

Embodiment 1

[0046] A method for extracting berberine from Phellodendron phellodendri, comprising the steps:

[0047] (1) Preparation of Phellodendron: Clean and sort Phellodendron, remove impurities, mildew and insects, and then pulverize to 60 mesh for use;

[0048] (2) Preparation of fermented bacterial liquid: The purified Lactobacillus plantarum and Clostridium butyricum were inserted into a conical flask from a slant, and cultured with shaking at 37° C. for 20 hours. The mixing ratio of bacterial liquid is: Lactobacillus plantarum 30%, Clostridium butyricum 20%;

[0049] (3) Preparation of culture medium: the components of culture medium include soybean meal powder, dimethyl hydrogen phosphate, glucose and water.

[0050] (4) Microbial fermentation: fully mix 100 parts of Cortex Phellodendri, 3 parts of probiotic liquid, 30 parts of soybean meal powder, 0.6 part of dimethyl hydrogen phosphate, 0.6 part of glucose and 180 parts of water with a three-dimensional mixer, put it into a c...

Embodiment 2

[0053] A method for extracting berberine from Phellodendron phellodendri, comprising the steps:

[0054] (1) Preparation of Phellodendron: Clean and sort Phellodendron, remove impurities and moldy, worm-bearing, and then pulverize to 24 meshes for later use;

[0055] (2) Preparation of fermented bacterial liquid: The purified Clostridium butyricum and Bacillus coagulans were inserted into a conical flask from a slant, and cultured with shaking at 30° C. for 30 hours. The mixing ratio of bacterial liquid is: Clostridium butyricum 25%, Bacillus coagulans 30%;

[0056] (3) Preparation of culture medium: the components of culture medium include soybean meal powder, dimethyl hydrogen phosphate, glucose and water.

[0057] (4) Microbial fermentation: fully mix 80 parts of Cortex Phellodendri, 2 parts of probiotic liquid, 80 parts of soybean meal powder, 0.5 part of dimethyl hydrogen phosphate, 0.5 part of glucose and 250 parts of water with a three-dimensional mixer, put it into a ...

Embodiment 3

[0060] A method for extracting berberine from Phellodendron phellodendri, comprising the steps:

[0061] (1) Preparation of Phellodendron: Clean and sort Phellodendron, remove impurities and moldy, worm-bearing, and then pulverize it to 80 mesh for use;

[0062] (2) Preparation of fermented bacterial liquid: The purified Lactobacillus plantarum, Lactobacillus bulgaricus and Hansenula yeast were inserted into a conical flask from a slant, and cultured with shaking at 40° C. for 22 hours. The mixing ratio of bacterial liquid is: Lactobacillus plantarum 20%, Lactobacillus bulgaricus 25%, Hansenula yeast 25%;

[0063] (3) Preparation of culture medium: the components of culture medium include soybean meal powder, dimethyl hydrogen phosphate, glucose and water.

[0064] (4) Microbial fermentation: Mix 90 parts of Cortex Phellodendri, 2 parts of probiotic liquid, 100 parts of soybean meal powder, 0.7 part of dimethyl hydrogen phosphate, 0.7 part of glucose and 230 parts of water wi...

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Abstract

The invention discloses a method for extracting berberine from cortex phellodendri chinensis, and relates to the technical field of traditional Chinese medicine extraction. The method includes the steps that the cortex phellodendri chinensis is subjected to microbial fermentation through composite probiotics, and then a fermentation product is subjected to water extraction to obtain an extract, wherein the composite probiotics include at least two of lactobacillus plantarum, lactobacillus bulgaricus, clostridium butyricum, hansenula polymorpha and bacillus coagulans. The cortex phellodendri chinensis is subjected to microbial fermentation pretreatment through the composite probiotics, mucilage in the cortex phellodendri chinensis can be decomposed by multiple enzymes generated by the compound probiotics in the fermentation process, thus the problem of difficult filtration in the traditional water extraction process of the cortex phellodendri chinensis is solved, and meanwhile, cell walls of the cortex phellodendri chinensis can be further decomposed, so that the berberine is easier to release; and in addition, acid generated during fermentation enables the fermentation product to be in an acid environment, the solubility of the berberine in water is increased, the extraction rate of the fermentation product can be increased, and the extraction effect is better.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a method for extracting berberine from Phellodendron chinensis. Background technique [0002] Phellodendron is the dry bark of the rutaceae plant, Phellodendron, and is one of the commonly used traditional Chinese medicines. Its main component is berberine. Studies have shown that Phellodendron of different grades, different production areas and harvesting periods, the content of berberine is not high. Similarly, Wu Shanshan's "Study on the Content of Berberine and Phellodendron in Different Grades of Treated Pieces of Treated Phellodendron" reported that the content of berberine in the first class decoction pieces was 6.4803-8.7309%, and the range of berberine in the second class decoction pieces was 3.7038-5.2703%. [0003] Berberine is a quaternary ammonium alkaloid, its free base is yellow needle-like crystals, slightly soluble in water, solubl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D455/03
CPCC07D455/03
Inventor 檀新珠丁扬洲陈新瑶梁世仁吴有林
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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