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A kind of fluorescent sericin platinum nanocluster and its preparation method and application

A platinum nanometer and fluorescent wire technology, applied in nanotechnology, nanotechnology, fluorescence/phosphorescence, etc., can solve the problems of long time, expensive instruments, cumbersome pre-processing, etc., and achieve less sample consumption, low detection cost, and production method. The effect of simple science

Active Publication Date: 2022-03-15
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, methods such as high performance liquid chromatography, enzyme-linked immunosorbent assay, and enzyme inhibition method used for the detection of pyrithion pesticide residues have defects such as cumbersome pre-treatment, expensive instruments, and long time-consuming.

Method used

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  • A kind of fluorescent sericin platinum nanocluster and its preparation method and application
  • A kind of fluorescent sericin platinum nanocluster and its preparation method and application
  • A kind of fluorescent sericin platinum nanocluster and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: Preparation of Sericin-Pt NCs

[0038] (1) Take 250 mg of sericin and add 5 mL of pure water to prepare a 50 mg / mL sericin solution. Take 5 mL of this solution and add 1 mL of 25 mM H 2 PtCl 6 Aqueous solution, vortex to mix thoroughly for 5min.

[0039] (2) Add 1M NaOH solution to the solution in the previous step to make the pH value 11. Add water to make up to 10mL, and vortex to mix again for 5min.

[0040] (3) Place the EP tube in a water bath at 60°C for 12 hours in the dark to obtain a polymer of platinum nanoclusters, centrifuge at 5,000 rpm for 10 minutes, and take the supernatant and store it in a refrigerator at 4°C in the dark for future use.

Embodiment 2

[0042](1) Take 125 mg of sericin and add 5 mL of pure water to prepare a 25 mg / mL sericin solution. Take 5 mL of this solution and add 1 mL of 50 mM H 2 PtCl 6 Aqueous solution, vortex to mix well for 10min.

[0043] (2) Add 1M NaOH solution to the solution in the previous step to make the pH value 10. Add water to make up to 10mL, and vortex to mix again for 5min.

[0044] (3) Place the EP tube in a water bath at 37°C for 8 hours in the dark to obtain a polymer of platinum nanoclusters, centrifuge at 5,000 rpm for 10 minutes, and take the supernatant and store it in a refrigerator at 4°C in the dark for future use.

Embodiment 3

[0046] (1) Take 250 mg of sericin and add 5 mL of pure water to prepare a 50 mg / mL sericin solution. Take 5 mL of this solution and add 1 mL of 100 mM H 2 PtCl 6 Aqueous solution, vortex to mix well for 10min.

[0047] (2) Add 1M NaOH solution to the solution in the previous step to make the pH value 12. Add water to make up to 10mL, and vortex to mix again for 5min.

[0048] (3) Place the EP tube in a 50°C water bath for 16 hours in the dark to obtain a polymer of platinum nanoclusters, centrifuge at 5,000 rpm for 10 minutes, and take the supernatant and store it in a 4°C refrigerator in the dark for future use.

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Abstract

The invention provides a sericin-wrapped blue-luminescent platinum nano-cluster and a preparation method and application thereof. The preparation method of the present invention is to mix the sericin protein and the high-priced platinum complex in a certain ratio, and after fully mixing with a vortex, add NaOH to adjust the pH value, and mix again with a vortex. Water bath for several hours to obtain a polymer of platinum nanoclusters. The preparation method of the present invention is simple in process, green, efficient, high in feasibility, and has extensive and practical application value. The nanocluster of the present invention can be applied to the detection of pyrithione in substances, and the application scope includes environmental water samples, soil samples, and fruit and vegetable samples; it can also be applied to the detection of chloramphenicol content in samples. The detection method of the invention is simple and efficient, has strong specificity and high sensitivity, and also provides a new idea for the use of sericin.

Description

technical field [0001] The invention belongs to the field of preparation and application of functional nano-fluorescent materials, and in particular relates to a fluorescent sericin platinum nano-cluster and its preparation method and application. Background technique [0002] Metal nanoclusters are fluorescent, water-soluble molecular aggregates composed of several to tens of atoms. Due to its unique quantum size effect, biocompatibility, strong photostability, and strong anti-polymerization ability, it has broad application prospects in the fields of biomarkers and environmental monitoring. Among them, platinum nanoclusters have many advantages such as good fluorescence properties, photostability, low toxicity, biocompatibility, and easy surface modification, and have attracted more and more attention from researchers. However, it is difficult to synthesize platinum nanoclusters with strong photostability, good biocompatibility, and simple and easy repeatability. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/102B22F1/054B22F9/24G01N21/64B82Y30/00B82Y40/00
CPCB22F9/24G01N21/6428B82Y30/00B82Y40/00G01N2021/6432B22F1/0553B22F1/07B22F1/102B22F1/054
Inventor 王宝娟常欣高鹏吴爽李全发鲁山陈杰熊声麒吴雨芬马晓燕
Owner ANHUI NORMAL UNIV
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