Fry breeding method of tetraploid fry of brachymystax lenok

Abstract

The invention relates to a fry breeding method of tetraploid fry of brachymystax lenok. The method comprises the following steps of firstly, performing in-vitro fertilization on eggs and sperms of parent brachymystax lenok; then, treating the fertilized eggs by means of a heat shock method; then, carrying out temperature change treatment on the fertilized eggs; finally, incubating the fertilized eggs in water for breeding, and performing culturing to obtain the tetraploid fry of brachymystax lenok. According to the method, the preparation of the tetraploid fry of brachymystax lenok is realizedby adopting an in-vitro fertilization technology for female and male brachymystax lenok and the heat shock method, the eyed rate, fry hatchability rate and tetraploid rate of the fertilized eggs areeffectively increased, and a stable triploid brachymystax lenok population can be obtained through hybridization with normal diploid brachymystax lenok, so that the problems of sexual prematurity, individual miniaturization and the like of bred individuals, which are caused by long-term inbreeding and lack of systematic breeding in a brachymystax lenok breeding process, are solved. The method increases the specification and growth speed of commercial fishes obtained through brachymystax lenok breeding and production and has good economic benefits and a high market value.

Description

technical field [0001] The present invention relates to the technical field of leptinus tetraploid seedlings, in particular to a seedling raising method of leptinus tetraploid seedlings. Background technique [0002] Thin scale salmon is named for its tiny scales. Salmonformes, Salmonidae, Lepidoptera. Commonly known as: mountain fine scale salmon, river fine scale salmon (Northeast), Lu fish, Lu flower fish, gold board fish, flower fish (Shaanxi), plum blossom fish (Gansu), small red fish (Xinjiang). It is an important economic fish in the Heilongjiang River Basin. The meat is plump, with high fat content (3.8-7.7%) and large body, generally weighing 1 kg, and the largest individual can reach 8 kg. In addition, eggs are also quite valuable. [0003] Studies have shown that salmon trout triploids can use all energy for somatic cell growth, thereby increasing the feed conversion rate. Triploids generally grow faster than diploids at the juvenile and mature stages, avoidin...

AI Technical Summary

Problems solved by technology

Although the existing technology for preparing triploid leptospirosis can obtain a very high triploid rate, its eyesight rate usually does not exceed 10%, and the seedling deformity rate is high, so it has not been able to form large-scale production. The reason is In the prior art, physical or chemical methods are used to treat the fertilized eggs, and the triploid seedlings prepared by hindering the second pole excretion cannot reach the triploid rate required for production or cannot reach the survival of the seedlings that can be used for production Rate