Xylosidase Xyl21 with high-concentration xylose, alcohol and salt tolerance as well as encoding gene and application of xylosidase Xyl21

Abstract

The invention relates to xylosidase Xyl21 with high-concentration xylose, alcohol and salt tolerance. An amino acid sequence of the xylosidase Xyl21 is SEQ ID NO.1. The optimum temperature of the xylosidase Xyl21 disclosed by the invention is 45 DEG C; after the xylosidase Xyl21 is treated at 45 DEG C for 1 h, the residual enzyme activity is 80%, the optimal pH is 5.5, the enzyme activity is keptat 80% or above in terms of relative enzyme activity within the pH range of 5.0-6.5, and the xylose tolerance, the ethanol tolerance and the tolerance to high-concentration salt ions are good. The xylosidase Xyl21 is novel and can tolerate high-concentration xylose, ethanol and salts, and due to the properties, the xylosidase Xyl21 serving as a novel enzyme preparation can be widely applied to industries such as energy, feed and food.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a xylosidase Xyl21 with high-concentration xylose, alcohol and salt tolerance, its coding gene and application. Background technique [0002] Hemicellulose is the main component of plants, and it can be converted into more valuable products through the degradation of biological enzymes. The main component of hemicellulose is xylan, which is a kind of heterogeneous polysaccharide linked by 1,4-glycosidic bonds and with multiple substituents. Its complete degradation is a complex process, in which xylosidase is the key enzyme to degrade xylan, and it hydrolyzes xylan from the non-reducing end in an exolytic way to finally produce xylose. However, in the process of industrial reaction, excessive xylose, as the end product, will have a feedback inhibition effect on xylosidase, thereby inhibiting the activity of xylosidase, and finally leading to the loss of its activity....

AI Technical Summary

Problems solved by technology

The current method for removing inhibition is to eliminate the influence of inhibitor xylose by adding enzyme dosage or ultrafiltration method, but this undoubtedly increases the cost of industrial production, so xylosidase with high concentration xylose tolerance can improve production efficiency to reduce costs; at the same time, in practical industrial applications, xylosidase with the characteristics of tolerance to high concentrations of ethanol can improve the production efficiency of ethanol, and xylosidase with the characteristics of tolerance to high concentrations of salt ions can be applied in salty biscuits In the food processing industry with high salt content such as bread, seafood, and sewage treatment, it is urgent to discover xylosidases with high concentrations of xylose, ethanol, and salt tolerance

Images