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Compound biocontrol microbial agent as well as preparation method and application thereof

A biocontrol agent and related technologies are applied in the field of compound biocontrol agents and their preparation, which can solve the problems of unstable control effect of a single bacterial species and a narrow range of antibacterial spectrum, and achieve comprehensive and efficient control effect and antibacterial effect. The effect of broad spectrum and low production cost

Pending Publication Date: 2019-11-29
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented inventor describes an organically produced bioremediation composition that contains both trichodermas (Trichodes) and glirocadiums(Gliocyllers). It was found effective against various types of harmful microorganisms like grey molds or root rots. Its main technical effects include promoter activity for producing higher levels of volatile substances called salty acids, increased cell size at roots, reduced water absorption into leaves, improved germination rates, longer life span without being affected by other factors affecting its performance, ability to prevent damage during storage over periods of several months, use in crops to decrease their susceptibility towards pests, reducing costs while maintaining good quality products.

Problems solved by technology

The technical problem addressed in this patented study relating to controlling vegetative diseases caused by botrytis caterpillars (Botrytis spp.) involves finding new ways to prevent these disease outbreaks while also maintaining their beneficial properties such as reducing damage from insect pests during storage periods without harmful side effects.

Method used

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  • Compound biocontrol microbial agent as well as preparation method and application thereof
  • Compound biocontrol microbial agent as well as preparation method and application thereof
  • Compound biocontrol microbial agent as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0037] This embodiment provides a kind of preparation method of compound biocontrol agent, comprises the following steps:

[0038] Step 1, taking bran, rice bran, and wheat grains to prepare a solid fermentation medium according to a certain mass ratio, and steam pressure sterilization;

[0039] Step 2, inoculate the fermented seed liquid of Trichoderma harzianum or the fermented seed liquid of G. pink in the sterilized solid fermentation medium prepared in step 1, ferment at room temperature for 15 days, and keep shaking the solid fermentation medium horizontally once a day;

[0040] Step 3. Spread the fermented product obtained from the fermentation in step 2 on a sterile tray, cover the fermented product with sterile filter paper, and stir the fermented product in the tray every day for 10 days. Completely dry, pulverize and sieve the fermented product to obtain Trichoderma harzianum powder or Myxomyces pink powder, respectively take Trichoderma harzianum powder or Myxomyce...

Embodiment 2

[0043] This embodiment provides a kind of preparation method of compound biocontrol agent, comprises the following steps:

[0044] Step 1. Take bran, rice bran and wheat grains to prepare 40g of solid fermentation medium according to the mass ratio of 1.67:1:7.23, place it in a tissue culture bottle, and shake it well; add water to the tissue culture bottle to make the medium initial The water content is 75%, sealed with two layers of sealing film, and allowed to stand at room temperature for 1 hour to allow the fermentation material to fully absorb water, then put the tissue culture bottle into a steam pressure cooker at 121°C and 20 psig for 30 minutes to sterilize, and wait until the pressure in the pressure cooker drops to Take it out from the pot at zero o'clock, shake it horizontally to avoid agglomeration, then let it cool at room temperature, and finally repeat the sterilization 3 times according to the above method;

[0045] Step 2. Inoculate the fermented seed liquid...

Embodiment 3

[0048] The present embodiment provides a compound biocontrol agent, comprising a certain volume ratio of Trichoderma harzianum suspension and Muxomyces pink suspension, wherein the living bacteria of Trichoderma harzianum suspension and Myxomyces pink suspension number is 10 6 cfu / mL.

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Abstract

The invention relates to a compound biocontrol microbial agent as well as a preparation method and an application thereof, which belong to the technical field of microorganisms. In order to solve theproblems of unstable control effect and narrow antibacterial spectrum range of a single strain, the invention provides a compound biocontrol microbial agent which comprises a trichoderma harzianum suspension and a gliocladium roseum suspension in a certain volume ratio, and the live bacteria number of the trichoderma harzianum suspension and the gliocladium roseum suspension is 106cfu/mL. The compound biocontrol microbial agent provided by the invention is wide in antibacterial spectrum, strong in antagonism, long in lasting period, low in production cost and simple in fermentation process, and can prevent and treat crop diseases caused by fungi such as botrytis cinerea, stem rot germs, rhizoctonia solani, anthracnose germs, fusarium and fusarium oxysporum when being applied to agricultural biological prevention and treatment; the microbial agent has a growth-promoting effect on crop seeds and seedlings, can promote seed germination, radicles and seedling growth, also has preservativeand fresh-keeping effects, and can reduce rotting rate and weight loss when applied to preservative and fresh-keeping of fruits and vegetables.

Description

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Claims

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Application Information

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Owner NORTHEAST AGRICULTURAL UNIVERSITY
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