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Single nucleotide polymorphism (SNP) molecular marker associated with pH and dropping loss character of pig muscles and application of SNP molecular marker

A molecular marker, drip loss technology, used in recombinant DNA technology, microbial assay/inspection, biochemical equipment and methods, etc.

Active Publication Date: 2019-11-19
湖南省畜牧兽医研究所
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Problems solved by technology

However, the regulatory mechanism of KLF5 in skeletal muscle has not been reported

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  • Single nucleotide polymorphism (SNP) molecular marker associated with pH and dropping loss character of pig muscles and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker associated with pH and dropping loss character of pig muscles and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker associated with pH and dropping loss character of pig muscles and application of SNP molecular marker

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Embodiment Construction

[0023] The present invention will be further explained below in conjunction with specific examples, but the specific implementation does not limit the present invention in any way.

[0024] The invention provides a SNP molecular marker related to pig muscle pH and drip loss traits. The nucleotide sequence of the SNP molecular marker is a specific DNA fragment cloned from the exon region of the pig KLF5 gene. The DNA fragment has a total of 528bp , wherein both the 160bp and 436bp of the sequence are A / G mutations.

[0025] Simultaneously, the present invention also provides a kind of detection method, is to detect two base mutation sites that exist on the 528bp amplified fragment of pig KLF5 gene, one is at the 160bp place of the target fragment, and the other is at the 436bp place of the target fragment, both It is an A / G mutation, and there is a correlation between the two mutations. The genotype of the pig is determined by the base, and then the meat quality traits are dete...

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Abstract

The invention belongs to the technical field of pig molecular marker preparation, and relates to a single nucleotide polymorphism (SNP) molecular marker associated with the pH and a dropping loss character of pig muscles and application of the SNP molecular marker. The SNP molecular marker nucleotide sequence is an obtained specific DNA fragment obtained by cloning from a second exon area of a pigKLF5 gene, and the DNA fragment has 528 bp, wherein A / G mutations are found at the 160 bp and the 436 bp in the sequence correspondingly. According to the SNP molecular marker associated with the pHand the dropping loss character of the pig muscles and application of the SNP molecular marker, the specific DNA fragment is obtained by cloning from the exon area of the pig KLF5 gene, SNP sites arelooked for, a corresponding detection method is established, and the new molecular marker is provided for selection and breeding of pork quality traits of pigs.

Description

technical field [0001] The invention belongs to the technical field of pig molecular marker preparation, and relates to a SNP molecular marker related to pig muscle pH and drip loss traits and its application, in particular to a single nucleotide polymorphic site of pig KLF5 gene as a pig pH and Molecular markers related to drip loss traits and their applications. Background technique [0002] Since the reform and opening up, my country's pig breeding has made remarkable achievements, the growth rate of commercial pigs has continued to accelerate, and the carcass lean meat rate has continued to increase. But what followed was a rough taste of the meat and a decline in muscle quality. With the improvement of people's living standards and the transformation of nutritional concepts, my country's pork market is developing towards the demand for high-quality meat. Therefore, breeding better high-quality meat pigs is an important issue before breeding scientists. [0003] Pork ...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 陈晨崔清明张星罗璇谢菊兰邓缘朱吉任慧波胡雄贵刘莹莹左剑波彭英林
Owner 湖南省畜牧兽医研究所
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