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a ceo-based 2 @sns 2 Facilitated preparation method of luminol electrochemiluminescence sensor

An electrochemistry and sensor technology, applied in the fields of biosensing and new nanomaterials, can solve the problems of poor reproducibility and low sensitivity, and achieve the effects of low cost, high sensitivity and simple preparation.

Active Publication Date: 2021-08-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, electrochemical biosensors are mainly used to detect procalcitonin, but this method has the disadvantages of poor reproducibility and low sensitivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. A kind of based on CeO 2 @SnS 2 A method for promoting the preparation of luminol electrochemiluminescent sensors, comprising the following steps:

[0027] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0028] (2) Drop-coat 6 μL of CeO with a concentration of 2 mg / mL on the surface of the glassy carbon electrode 2 @SnS 2 The material solution is the sensing substrate, which is placed at 37 °C to dry;

[0029] (3) Add 6 μL of antibody-labeled luminol-ferritin (Ft-luminol) solution at a concentration of 2 mg / mL dropwise, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it at 4 °C C to dry;

[0030] (4) Add 3 μL bovine serum albumin solution with a mass fraction of 1% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer solu...

Embodiment 2

[0032] Embodiment 2. A kind of based on CeO 2 @SnS 2 A method for promoting the preparation of luminol electrochemiluminescent sensors, comprising the following steps:

[0033] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0034] (2) Drop-coat 6 μL of CeO with a concentration of 3 mg / mL on the surface of the glassy carbon electrode2 @SnS 2 The material solution is the sensing substrate, which is placed at 37 °C to dry;

[0035] (3) Add 6 μL of antibody-labeled luminol-ferritin (Ft-luminol) solution at a concentration of 3 mg / mL dropwise, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it at 4 °C C to dry;

[0036] (4) Add 3 μL bovine serum albumin solution with a mass fraction of 2% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer soluti...

Embodiment 3

[0038] Embodiment 3. A kind of based on CeO 2 @SnS 2 A method for promoting the preparation of luminol electrochemiluminescent sensors, comprising the following steps:

[0039] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0040] (2) Drop-coat 6 μL of CeO with a concentration of 4 mg / mL on the surface of the glassy carbon electrode 2 @SnS 2 The material solution is the sensing substrate, which is placed at 37 °C to dry;

[0041] (3) Add 6 μL of antibody-labeled luminol-ferritin (Ft-luminol) solution at a concentration of 4 mg / mL dropwise, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it at 4 °C C to dry;

[0042] (4) Add 3 μL bovine serum albumin solution with a mass fraction of 3% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer solu...

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Abstract

The invention relates to a CeO-based 2 @SnS 2 The preparation method of promoting luminol electrochemiluminescence sensor belongs to the field of new nanomaterials and the field of biosensing technology; the present invention is based on electrochemiluminescence ECL technology, and for the first time uses ferritin covalently cross-linked luminol (Ft-luminol ) as the signal source, with CeO 2 @SnS 2 As an accelerator, using CeO 2 @SnS 2 The excellent synergistic catalytic effect of luminol effectively amplifies the detection signal, and a biosensor preparation method with simple preparation, low cost, low reaction energy consumption, and green environmental protection is proposed, and it is applied to the actual production of procalcitonin For sample detection, the detection limit is as low as 1.6 fg / mL, and the linear range is as wide as 5 fg / mL‑100 ng / mL. It has high sensitivity and good reproducibility, and has great potential application value.

Description

technical field [0001] The invention belongs to the field of novel nanomaterials and the field of biosensing technology Background technique [0002] As a research hotspot arising from the intersection of multiple disciplines such as biology, chemistry, medicine, and electronic technology, electrochemiluminescence (ECL) immunoassay technology is an organic combination of electrochemistry, chemiluminescence, and immunoassay technologies. It has the advantages of low cost, good selectivity, high sensitivity, fast analysis speed, easy automation, miniaturization and integration, and has been widely used in disease marker analysis, food safety analysis, environmental pollution analysis and other fields. [0003] Procalcitonin is a disease marker for diagnosing sepsis and identifying severe bacterial infections. Its sensitive detection can significantly improve the sensitivity and specificity of sepsis diagnosis. At present, electrochemical biosensors are mainly used to detect p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/327G01N27/30
CPCG01N21/76G01N27/308G01N27/3275
Inventor 杨磊魏琴冯锐刘蕾刘雪静张诺马洪敏
Owner UNIV OF JINAN
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