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Sampling and staining method of epidermis fungi

A dyeing method, fungal technology, applied in the direction of sampling, test sample preparation, instruments, etc., can solve the problems of easy loss of samples, difficult dyeing operation, difficult sampling, etc., to achieve improved success rate and efficiency, short dyeing time, The effect of easy sampling

Active Publication Date: 2021-02-26
QUEENS SHENZHEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current problems in sampling and dyeing of epidermis fungi such as difficult sampling, easy contamination of samples, difficult dyeing operation, easy loss of samples, and difficulty in distinguishing dander and fungi, the invention provides a method for sampling and dyeing of epidermis fungi

Method used

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  • Sampling and staining method of epidermis fungi
  • Sampling and staining method of epidermis fungi
  • Sampling and staining method of epidermis fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for sampling and staining epidermis fungi, comprising the following steps:

[0046] (1). A transparent detection card made of glass is provided. The transparent detection card has a detection area. The detection area is attached with a layer of acrylic layer. The acrylic layer is circular, with a thickness of 1mm and an area of ​​153.86mm 2 , the surface of the acrylic layer is covered with a protective film;

[0047] (2). Use a sterilized sampling comb to comb along the hair direction of the person to be tested 3 times, and comb different parts of the hair each time, so that dander sticks to the sampling comb;

[0048] (3). Remove the protective film on the glass material transparent detection card, stick the tip of the sampling comb with dander on the surface of the acrylic layer, and place the other parts of the sampling comb with dander parts stick to the surface of the acrylic layer such that a sufficient amount of dander adheres to the acrylic layer;

...

Embodiment 2

[0054] A method for sampling and staining epidermis fungi, comprising the following steps:

[0055] (1). Provide a transparent detection card made of glass. The transparent detection card has a detection area, and a layer of acrylic layer is attached to the detection area. The acrylic layer is circular, with a thickness of 0.5mm and an area of ​​346.18mm 2 , the surface of the acrylic layer is covered with a protective film;

[0056] (2). Use a sterilized sampling comb to comb along the hair direction of the person to be tested 3 times, and comb different parts of the hair each time, so that dander sticks to the sampling comb;

[0057] (3). Remove the protective film on the transparent detection card made of glass, stick the tip of the sampling comb with dander on the surface of the acrylic layer, and place the other parts of the sampling comb with dander parts stick to the surface of the acrylic layer such that a sufficient amount of dander adheres to the acrylic layer;

[...

Embodiment 3

[0062] A method for sampling and staining epidermis fungi, which is detected by mailing a sample comb in this embodiment.

[0063] (1). Sample acquisition: because the subject is not in the local area, it is inconvenient to test on the spot. By sending a clean and disinfected sample comb, the subject himself combs the scalp with the sample comb according to the operating instructions of the present invention and then combs the sample. Put the comb into a sterile sealed bag, store it at room temperature, and mail it to the testing laboratory for testing within one week.

[0064] (2). Provide a transparent detection card made of glass, which has a detection area, and a layer of acrylic layer is attached to the detection area. The acrylic layer is circular, with a thickness of 1.5mm and an area of ​​78.5mm 2 , the surface of the acrylic layer is covered with a protective film;

[0065] (3). Remove the protective film on the transparent detection card made of glass, stick the tip...

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Abstract

The invention provides a method for sampling and dyeing epidermis fungi. The method for sampling and staining epidermis fungi comprises: providing a transparent detection card with a detection area, the detection area is attached with a layer of transparent viscous material layer, and the surface of the transparent viscous material layer is covered with a protective film; Comb several times in the direction of the patient's hair to make the dander stick to the sampling comb; remove the protective film on the transparent test card, and repeatedly stick the tip of the sampling comb with dander on the surface of the transparent viscous material layer to make enough The dander sticks on the transparent viscous material layer; the same amount of A dye solution and B dye solution are sequentially dripped on the surface of the transparent viscous material layer, and rinsed with deionized water after standing; transfer the transparent detection card to a microscope for microscopy check. The method has the characteristics of convenient sampling, easy transfer of the sample, not easy contamination, easy fixation of the sample, short dyeing time, etc., and improves the success rate and efficiency of sampling dyeing.

Description

technical field [0001] The invention belongs to the technical field of fungal detection and treatment, and in particular relates to a method for sampling and dyeing epidermis fungi. Background technique [0002] Epidermal fungal detection plays an important guiding role in the diagnosis and prevention of skin and hair diseases. Among them, direct sampling, staining and microscopic examination is one of the fastest judgment methods, and can also provide key guidance information for further culture identification. [0003] The traditional epidermis sampling method is to use a scalpel or the end of a glass slide to scrape off the skin debris at the test site, collect it in an appropriate package, then transfer it to the laboratory, and stain it on a glass slide for microscopic examination. This method of sampling and dyeing has the following defects: (1) blades or glass slides are not suitable for sampling the skin on the head; (2) the scraped samples need to be transferred by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 禹洋江颖纯黎璐马敬信林焱吴玥马洁
Owner QUEENS SHENZHEN BIOTECH CO LTD
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