Tissue culture method for obtaining regenerated plantlet by using hippeastrum vittatum plateau as explant
A technology for regenerating plants and explants, applied in the field of seedling propagation of herbaceous ornamental plants, can solve the problems of unstable production and low proliferation rate, and achieve the effect of rapid reproduction and broad application prospects
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Embodiment 1
[0032] The four parts of Hippeastrum chinensis without bulb, bulb disk with bulb, base of inflorescence axis and top of inflorescence axis were used as explants to compare the pollution rate of different parts of explants and the effect on the induction of adventitious buds of Hippeastrum chinensis.
[0033] Disinfection methods are all adopted "shake with detergent solution for 15 minutes, rinse with running water for 30 minutes, disinfect with 75% ethanol on a sterile operating table for 60 seconds, then disinfect with 0.1% mercury chloride for 22 minutes, and finally wash with sterile water for 6 times. , wash 1min each time". Cultured in induction medium MS+6-BA 2 mg / L +NAA 0.1 mg / L. Each treatment received 100 explants, repeated 3 times, and the contamination rate, induction rate and induction multiple were counted after 45 days.
[0034] The test results are shown in Table 1. From Table 1, it can be seen that the pollination rate of the inflorescence rachis as an explan...
Embodiment 2
[0038] Bulb discs with bulbs were used as explants. After disinfection, they were inoculated on induction media containing different hormone ratios (CPPU, KT, BA and NAA), and the effects of different media on the induction of adventitious buds were compared. The development of explants was observed, and the adventitious bud induction rate and induction multiple were counted after 45 days.
[0039] It can be seen from Table 2 below that on the MS+6-BA 2mg / L+NAA0.1 mg / L medium, the explants developed best, the induction rate was the highest, and the number of induced adventitious buds was the largest.
[0040] Table 2 The effect of induction medium on the induction of adventitious buds of Lycoris safflower
[0041]
Embodiment 3
[0043] Hippeastrum is a perennial flower, and the explants taken are underground parts, so it is difficult to control the pollution rate. In order to reduce the contamination rate of explants, the effects of different combinations of disinfection methods on the contamination rate of explants were compared. Firstly, the pretreatment and disinfection methods were compared. The explants were treated with 84 disinfectant, Amway detergent, and a mixture of penicillin and streptomycin for 20 minutes, and three kinds of disinfectants were used for 10 minutes each for disinfection pretreatment. Then, transfer to the aseptic operating table, soak the explants in 75% alcohol for 60 seconds, and treat them with 0.1% mercury liter for 20 minutes to disinfect the explants. Secondly, to compare the mercury disinfection time, use the Amway detergent solution to shake and soak for 15 minutes, transfer it to the sterile operating table, and soak it in 75% alcohol for 60 seconds. And then trea...
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