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A kind of ephemeral plant Arabidopsis thaliana tissue culture method

A kind of Arabidopsis thaliana, tissue culture technology, applied in the field of plant tissue culture, can solve problems such as no report of Arabidopsis thaliana tissue culture technology

Active Publication Date: 2022-06-28
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many researchers use plant callus to conduct transgenic research, but the tissue culture technology of Arabidopsis thaliana has not been reported yet

Method used

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  • A kind of ephemeral plant Arabidopsis thaliana tissue culture method
  • A kind of ephemeral plant Arabidopsis thaliana tissue culture method
  • A kind of ephemeral plant Arabidopsis thaliana tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The seeds of Arabidopsis thaliana in Xinjiang were first washed with 70% ethanol for 1 min in an ultra-clean workbench, and then washed with 20% sodium hypochlorite solution for 8-10 min by shaking. Finally, wash 6-7 times with sterile water, fully shake and mix well each time. Seeds of Arabidopsis thaliana were sown on MS-containing petri dishes, placed in vernalization treatment at 4°C for 3 days, and then placed in a light incubator at 16h light / 8h dark at 22°C for 7- After 8 days, the seedlings of Arabidopsis thaliana were obtained, and the roots of the seedlings of Arabidopsis thaliana were taken as explants.

[0034] Cut the roots (about 1 cm) of young Arabidopsis thaliana seedlings grown for 8 days as explants. The cut explants were placed on the adventitious bud medium, and several explants were randomly placed in each dish, and the explants were transferred to fresh medium every week, and the callus induction and adventitious bud induction were observed, and p...

Embodiment 2

[0037]The seeds of Arabidopsis thaliana in Xinjiang were first washed with 70% ethanol for 1 min in an ultra-clean workbench, and then washed with 20% sodium hypochlorite solution for 8-10 min by shaking. Finally, wash 6-7 times with sterile water, fully shake and mix well each time. Seeds of Arabidopsis thaliana were sown on MS-containing petri dishes, placed in vernalization treatment at 4°C for 3 days, and then placed in a light incubator at 16h light / 8h dark at 22°C for 7- After 8 days, small Arabidopsis seedlings were obtained, and the hypocotyls of the small Arabidopsis seedlings were taken as explants.

[0038] The hypocotyls (about 1 cm) of Arabidopsis thaliana seedlings grown for 8 days were clipped as explants. The cut explants were placed on the adventitious bud medium (MS medium+6-BA (0.5mg / L)+NAA (0.1mg / L)), and several explants were randomly placed in each dish, and the The explants were transferred to a fresh medium, and the callus induction and adventitious b...

Embodiment 3

[0041] The seeds of Arabidopsis thaliana in Xinjiang were first washed with 70% ethanol for 1 min in an ultra-clean workbench, and then washed with 20% sodium hypochlorite solution for 8-10 min by shaking. Finally, wash 6-7 times with sterile water, fully shake and mix well each time. Seeds of Arabidopsis thaliana were sown on MS-containing petri dishes, placed in vernalization treatment at 4°C for 3 days, and then placed in a light incubator at 16h light / 8h dark at 22°C for 7- The seedlings of Arabidopsis thaliana were obtained in 8 days, and the seedlings of Arabidopsis thaliana in good growth condition were transplanted into jars containing MS medium, and then placed in an incubator under the same culture conditions for about 4 weeks. Petioles serve as explants.

[0042] Cut the petiole (about 1 cm) of Arabidopsis thaliana about 4 weeks old as an explant. The cut explants were placed on the adventitious bud induction medium (MS medium+6-BA (0.5mg / L)+NAA (0.1mg / L)), and se...

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Abstract

The invention belongs to the field of plant tissue culture methods, in particular to a method for tissue culture of ephemeral plant Arabidopsis xinjiang. The described Xinjiang small Arabidopsis tissue culture method comprises the following steps: 1) disinfection of Xinjiang small Arabidopsis seeds, 2) the sterilized seeds are sown on-demand and cultivated on the medium; Select explants on Arabidopsis thaliana seedlings; 4) carry out callus induction culture on explants in adventitious bud medium, obtain callus; further carry out adventitious bud induction culture to obtain adventitious buds; 5) culture adventitious buds on rooting Rooting induction culture was carried out to obtain whole plants. The invention provides tissue culture of different explants of Arabidopsis thaliana, preliminarily establishes the tissue culture technology of different explants of Arabidopsis thaliana, and provides a certain research basis for subsequent research on gene transformation and functional identification of Arabidopsis thaliana. To provide a theoretical basis for the further exploration of stress-tolerant resources and environmental adaptability of Arabidopsis thaliana.

Description

technical field [0001] The invention belongs to the field of plant tissue culture methods, in particular to a method for tissue culture of ephemeral plant Arabidopsis xinjiang. Background technique [0002] Arabidopsis pumila is an early spring ephemeral plant, mainly distributed in the extreme environment of Xinjiang. Arabidopsis thaliana is a close relative of the model plant Arabidopsis thaliana, which also belongs to the Brassicaceae. And compared with Arabidopsis, it has stronger salt and drought resistance, stronger photosynthetic efficiency and larger fruit volume, and its plant type is larger than Arabidopsis. It has been reported that Arabidopsis japonicus can adapt to arid environments and tolerate a certain degree of salt stress compared with Arabidopsis thaliana. It is a potential model material for studying the mechanism of biological and environmental adaptability, and also a model plant for studying the mechanism of rapid growth and development of ephemeral ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄先忠金玉环张亭亭刘丹青
Owner SHIHEZI UNIVERSITY
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