Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of construction method of afft1 cell

A cell and precise technology, applied in the field of AFFT1 cells and its preparation, can solve the problems of not considering the self-protection technology of T cells, and achieve the effect of improving lethality

Active Publication Date: 2021-03-16
BEIJING DCTY BIOTECH CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] None of the above schemes consider the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful immune microenvironment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of construction method of afft1 cell
  • A kind of construction method of afft1 cell
  • A kind of construction method of afft1 cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0101] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention

[0102] 1. Whole Exome Sequencing

[0103] 1) Take peripheral blood from lung cancer patients for ctDNA sequencing and HLA typing;

[0104] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0105] 2. Epitope prediction

[0106] 1) Take the mutated amino acid site as the center, extend 10 amino acids to both sides, and use this 21-amino acid polypeptide as a "potential antigenic epitope";

[0107] 2) Use prediction software to analyze the IC50 of the potential antigenic epitope (recommended software: NetMHCpan 3.0, PickPocket, artificial neural networks (ANN)), if IC50<1000nM, the potential antigenic epitope is considered as "antigenic epitope";

[0108] 3. Synthetic peptides

[0109] Antigen epitope peptide synthesis method...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of AFFT1 cells. RFF cells are transformed through a TCR-T technical principle, then immunosuppression target spots of transformed T cells are knocked outthrough a gene editing technology, thus the specific cytotoxic T cells are precisely protected against being inhibited in vivo, and the lethality of the T cells on tumor cells is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an AFFT1 cell and a preparation method thereof. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proven to be ineffective, while NK, CAR-NK, TIL, and other cell technologies have yet to mature. CAR - T cells have shortcomings in safety and solid tumor therapy. [0003] In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0004] The above treatment methods are immature, especially the in vitro induction of DC ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/90C12N5/10
CPCC07K14/7051C12N5/0638C12N15/907C12N2510/00C12N2800/80C12N2810/10
Inventor 焦顺昌张嵘周子珊解佳森陈小彬陈红利
Owner BEIJING DCTY BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com