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Minimal medium, pinellia ternate tissue culture medium and pinellia ternate tissue culture method

A basic culture medium and technology of culture medium, applied in the field of plant tissue culture, can solve the problems of inability to propagate fast and good shape, and low efficiency of fast propagation of Pinellia tissue culture, so as to improve the induced differentiation rate, improve production efficiency and production quality, The scientific and reasonable effect of the formula

Active Publication Date: 2019-10-29
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects in the prior art, to provide a kind of medium that can completely replace MS medium for pinellia tissue culture, to solve the problem of low efficiency of tissue culture and fast propagation of Pinellia pinellia, and the inability to rapidly propagate Pinellia pinellia with good shape The problem

Method used

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  • Minimal medium, pinellia ternate tissue culture medium and pinellia ternate tissue culture method
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  • Minimal medium, pinellia ternate tissue culture medium and pinellia ternate tissue culture method

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: the configuration of each mother solution of basic medium

[0030] Process S1:

[0031] 1. Weigh a large number of element components a: 2g of ammonium sulfate, 38g of potassium nitrate, 2g of potassium dihydrogen phosphate and 4g of magnesium sulfate, and then dissolve them in ultrapure water in turn and set the volume in a 1L volumetric flask. Invert the 1L volumetric flask to mix. After uniformity, pour it into a wide-mouth bottle for use, and make it into mother solution Ⅰ. When using, prepare 50ml of mother solution Ⅰ per liter of medium;

[0032] Weigh 10g of calcium ammonium nitrate, a large amount of element component b, dissolve it in ultrapure water and set the volume in a 1L volumetric flask, invert the 1L volumetric flask to mix well, pour it into a clean light bottle for later use, and prepare mother liquor II , prepare 50ml of mother solution II per liter of medium when used;

[0033] 2. Weigh trace element components: manganese sulfate tet...

Embodiment 2

[0050] Embodiment 2: Pinellia pinellia cluster bud induction medium test

[0051] processing 1

[0052] 1. Preparation of mother solutions of hormones in the induction medium of pinellia pinellia clustering buds

[0053] Weigh 100mg of naphthaleneacetic acid and put it into 2ml of sodium hydroxide solution with a concentration of 1mol / L, and after completely dissolving, dilute it with ultrapure water in a 100ml volumetric flask to obtain a naphthaleneacetic acid hormone mother solution with a concentration of 1mg / ml;

[0054] Weigh 100mg of 6-benzylaminopurine solution and dissolve it in 2ml of 1mol / L sodium hydroxide. After completely dissolving, dilute it with ultrapure water in a 100ml volumetric flask to obtain 6-benzylaminopurine with a concentration of 1mg / ml. Hormone mother liquor.

[0055] 2. Preparation of Pinellia Cluster Bud Induction Medium

[0056] Boil 500ml ultrapure water, get the consumption of each component mother liquor according to the processing S1 in ...

Embodiment 3

[0085] Embodiment 3: Pinellia pinelliae proliferating medium test

[0086] processing 1

[0087] 1. Preparation of mother liquors of various hormones in the proliferation medium of pinellia cypress buds

[0088] Weigh 100mg of naphthaleneacetic acid and put it into 2ml of sodium hydroxide solution with a concentration of 1mol / L, and after completely dissolving, dilute it with ultrapure water in a 100ml volumetric flask to obtain a naphthaleneacetic acid hormone mother solution with a concentration of 1mg / ml;

[0089] Weigh 100mg of 6-benzylaminopurine solution and dissolve it in 2ml of 1mol / L sodium hydroxide. After completely dissolving, dilute it with ultrapure water in a 100ml volumetric flask to obtain 6-benzylaminopurine with a concentration of 1mg / ml. Hormone mother liquor.

[0090] 2. Preparation of Proliferation Medium for Pinellia Cluster Buds

[0091] Boil 500ml ultrapure water, get the consumption of each component mother liquor according to the processing S1 in ...

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Abstract

The invention discloses a minimal medium, a pinellia ternate tissue culture medium and a pinellia ternate tissue culture method. The minimal medium comprises macroelement components A, macroelement components B, microelement components, organic element components and iron salt components. The pinellia ternate minimal medium based on the minimal medium by proportioning is established, and a pinellia ternate cluster bud induction culture medium, a pinellia ternate cluster bud proliferation culture medium and a pinellia ternate rooting culture medium which are prepared on the basis of the pinellia ternate minimal medium and are involved in the culture method are selected and optimized; the minimal medium has the advantages of high bud induction rate, high proliferation coefficient, high rooting rate, fast rooting, developed root system, thick plants, high transplanting survival rate and low cost, industrialized seedling cultivation of pinellia ternate tissue culture is achieved, and application and popularization are facilitated.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture in biotechnology, in particular to a basic culture medium, a Pinellia tissue culture medium and a Pinellia tissue culture method. Background technique [0002] Pinellia is a perennial herb of the family Araceae. Its tubers are used as medicine. It has the functions of drying dampness and reducing phlegm, reducing adverse reactions and relieving vomiting, and dispelling mass. It is an important traditional Chinese medicinal material. Undergrowth. As a widely used bulk Chinese herbal medicine, with the increasing market consumption, the wild resources of Pinellia chinensis are decreasing year by year, which can no longer meet the growing market demand. Artificial cultivation is the main way to obtain Pinellia pinellia. [0003] At present, artificial cultivation adopts small tubers to raise seedlings, which requires a large amount of seeds, high cost, and there are problems such as lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 舒福兴陈集双贾启萨尔赛亚·萨仁德勒周奇年陈忠文姜再璐李志雄谢欣
Owner ZUNYI MEDICAL UNIVERSITY
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