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Composite microbial agent for efficiently converting crop straw and preparation method and application of composite microbial agent

A technology of crop straw and compound bacterial agent, which is applied in the field of biological agriculture, can solve the problems such as the need to improve the transformation effect and the low recycling efficiency, and achieve the effects of stable transformation, good effect and organic matter improvement.

Pending Publication Date: 2019-10-22
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a compound microbial agent for efficiently transforming crop straws and its preparation method and application in view of the problems of low recycling efficiency of existing crop straw resources and improvement of transformation effect

Method used

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  • Composite microbial agent for efficiently converting crop straw and preparation method and application of composite microbial agent
  • Composite microbial agent for efficiently converting crop straw and preparation method and application of composite microbial agent
  • Composite microbial agent for efficiently converting crop straw and preparation method and application of composite microbial agent

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specific Embodiment approach 1

[0027] Specific Embodiment 1: In this embodiment, the compound microbial agent for efficiently transforming crop straws is formed by mixing composite bacterial flora and a fixative, and the composite bacterial flora is from 37% to 37% by mass. 43% Streptomyces grisea, 27%-33% Aspergillus niger and 27%-33% Trichoderma viride.

[0028] The fixative in the composite bacterial preparation of this embodiment is only used as a carrier for fixing the composite bacterial flora, and its content has little impact on the composite bacterial flora. The fixative content can be selected according to the routine in this field, such as accounting for 20% of the total mass of the composite bacterial preparation ~50%.

[0029] The Streptomyces griseorubens described in the compound bacterial agent of this embodiment is obtained by self-screening and identification, the described Aspergillus niger is obtained by self-screening and identification, and the Trichoderma viride (Trichoderma viride) C...

specific Embodiment approach 2

[0030] Specific embodiment two: what this embodiment is different from specific embodiment one is that described composite flora is made up of 40% Streptomyces grisea, 30% Aspergillus niger and 30% Trichoderma viridans by mass percentage .

specific Embodiment approach 3

[0031] Specific implementation mode three: the preparation method of the compound microbial agent for efficiently transforming crop straws in this embodiment is implemented according to the following steps:

[0032] 1. Inoculate Streptomyces grisea into the activation medium and carry out constant temperature cultivation at 26°C-28°C to obtain activated Streptomyces grisea. The components in the activated solid medium are: 3.0-5.0g / L yeast extract powder, 8.0~12.0g / L malt extract powder, 3.0~5.0g / L glucose and 16.0~20.0g / L agar;

[0033] Two, aspergillus niger is inoculated on the PDA medium and carries out activation culture, obtains the aspergillus niger of activation;

[0034] Three, Trichoderma viride is inoculated on the PDA medium and carried out activation culture, obtains the Trichoderma viride of activation;

[0035] 4. The activated Streptomyces grisea, Aspergillus niger and Trichoderma viride were purely cultured after 3 to 5 subcultures respectively, and Streptom...

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Abstract

The invention relates to a composite microbial agent for efficiently converting crop straw and a preparation method and application of the composite microbial agent and belongs to the technical fieldof biological agriculture. The problems are solved that in the prior art, the crop straw resource recycling utilization efficiency is low, and the conversion effect needs to be improved. The compositemicrobial agent for efficiently converting the crop straw is prepared by mixing composite flora and a fixing agent, and the composite flora is prepared from, by mass, 37-43% of streptomyces griseorubens, 27-33% of aspergillus niger and 27-33% of trichoderma viride. According to the application, the composite microbial agent is dissolved in water to prepare a composite microbial solution, and thesurface of the crop straw is sprayed with the composite microbial solution for conversion. In order to meet the growth demand of the composite flora in the continuous fermentation process, by generating cellulase and ligninase, all the components of the crop straw are converted to generate small molecule reducing sugar such as glucose and xylose. The selected composite flora can stably achieve a conversion function and has a good effect.

Description

technical field [0001] The invention belongs to the technical field of biological agriculture, and in particular relates to a microbial compound bacterial agent applied to the transformation of crop straws and a preparation method thereof, so as to realize resource utilization of crop straws. Background technique [0002] Our country is in the fast lane of rapid development of agricultural economy, with the characteristics of dense population and vast territory. The annual output of crop straw in my country is astonishing, reaching 800 million tons, ranking first in the world. Generally, crop straws are treated in the following ways: [0003] First: direct burning or direct disposal in the field. This method not only causes severe air pollution and severe smog, but also wastes valuable biomass raw materials. [0004] Second: return to the field through mechanical crushing or return the compost to the field after it is decomposed. However, due to the low efficiency and sl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12P19/02C05F11/08C05F17/00C12R1/465C12R1/685C12R1/885
CPCC12N1/20C12N1/14C12P19/02C05F11/08C05F17/20Y02W30/40
Inventor 杨谦林枞雨
Owner HARBIN INST OF TECH
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