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Competition-method-based homocysteine fluorescent immunochromatography detection kit and using method thereof

A technology of fluorescence immunochromatography and homocysteine, which is applied in biological testing, measuring devices, analytical materials, etc., can solve problems such as inability to realize rapid diagnosis, and achieve the effects of easy promotion, low cost, and strong specificity

Pending Publication Date: 2019-10-18
NANJING NANOEAST BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is simple to operate and low in cost, but requires the use of large-scale instruments such as automatic biochemical analyzers, and cannot achieve rapid diagnosis

Method used

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  • Competition-method-based homocysteine fluorescent immunochromatography detection kit and using method thereof
  • Competition-method-based homocysteine fluorescent immunochromatography detection kit and using method thereof
  • Competition-method-based homocysteine fluorescent immunochromatography detection kit and using method thereof

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1: kit preparation and detection method:

[0041] Kits for preparing competitive fluorescent immunochromatography test paper for detecting homocysteine ​​in human serum:

[0042] Reagent I: In 1L 10mmol / L PBS buffer, add 5mmol / L adenosylmethionine, 50KU / L homocysteine ​​methyltransferase, 10mmol / L dithiothreitol, 0.1% Sodium nitrogen, stir evenly, namely reagent I.

[0043] Reagent II: Add 100 mg of avidin fluorescent microspheres and 0.1% sodium azide to 1 L of 10 mmol / L PBS buffer, and stir well to become reagent II.

[0044] Test strip: its structure is as follows figure 1shown. On each 4 mm strip, 0.8 μL biotinylated adenosylhomocysteine ​​antibody on the conjugate pad, 0.4 μL adenosylhomocysteine-bovine serum albumin conjugate on the nitrocellulose membrane, 0.32 μL of secondary antibody.

[0045] The detection method is as follows:

[0046] Take the fluorescent immunochromatographic quantitative analyzer as an example: the sample volume is 20 μL, ...

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Abstract

The invention discloses a competition-method-based homocysteine fluorescent immunochromatography detection kit and a using method thereof. The kit comprises a reagent I, reagent II and a fluorescent immunochromatographic strip. The reagent I contains dithiothreitol (DTT), adenosylmethionine, homocysteine methyltransferase, a buffer solution, and a stabilizer. The reagent II contains a biotinylatedpolystyrene fluorescent microsphere, a buffer solution, and a stabilizer. The test strip includes a sample pad, a binding pad coated with a biotinylated antibody, an NC membrane containing a detection line and a quality control line, a piece of absorbent paper and a PVC rubber sheet. The competitive fluorescent immunochromatography is used for detecting the adenosine homocysteine, thereby detecting the homocysteine. The method is operated simply and is capable of detecting lots of samples; the interference factors are reduced; and the cost is low.

Description

technical field [0001] The invention relates to a homocysteine ​​fluorescent immunochromatography detection kit and a detection method thereof, belonging to the technical field of rapid in vitro diagnosis. Background technique [0002] Homocysteine ​​(hereinafter referred to as Hcy) is an important intermediate product in the metabolic process of methionine. Under disease conditions, the metabolism of the enzyme is impaired, and the concentration of Hcy in the blood increases. In 1932, Du Vigneaud discussed Hcy for the first time. In 1932, Carson and Neil discovered homocystinuria in children with congenital intellectual disabilities, and found that these patients were prone to thromboembolic diseases. In 1969, McCully first wrote the Hcy level. It may be related to atherosclerosis. In 1976, Wilcken et al. proposed that Hcy is an independent risk factor for cardiovascular diseases through epidemiological investigations. Since the 1990s, a large number of studies have been c...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/68G01N33/533
Inventor 张宇王建国韩国志
Owner NANJING NANOEAST BIOTECH
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