Universal antigen retrieval buffer solution

Abstract

The present invention discloses an universal antigen retrieval buffer solution, which is characterized in that: the buffer solution comprises citraconic anhydride 0.5 g / L; and nonionic surfactant 5ml / L; the preparing method of the buffer solution has following steps with preparation of one liter buffer solution as an example: weighing 0.5 g of citraconic anhydride, taking a 1000 ml beaker, dissolving 0.5 g of citraconic anhydride, dissolving in 800 ml of distilled water, stirring and mixing; adding 5 ml of nonionic surfactant to the mixed solution, stirring and mixing; adjusting pH valueto 7.4 with an NaOH solution of an equivalent concentration of 1N; then taking a 1000 ml graduated cylinder and pouring the solution with the pH value of 7.4 into the graduated cylinder and fixing volume to 1000 ml with distilled water. The universal antibody retrieval solution can be applied to most of antibodies using thermal retrieval for antigen retrieval, can greatly facilitate accurate application of immunohistochemical antigen retrieval methods for pathology and scientific research.

Description

technical field [0001] The invention relates to the field of biomacromolecular immune detection, is applied to immunohistochemical detection technology, and particularly relates to a universal antigen retrieval buffer. Background technique [0002] At present, formaldehyde is often used as a routine specimen fixative in clinical pathology departments or basic biomedical scientific research units. Although formaldehyde fixation can prevent the corruption and autolysis of the tissue after it is isolated from the body, maintain the original shape of the tissue cells, and better preserve the antigenicity of the biological macromolecules inside the tissue; however, the aldehyde group of formaldehyde and the amino group in the tissue protein The formation of cross-links will shield most of the antigenic determinants, resulting in the inability of the antibody to effectively combine with the target antigen, thus affecting the results of immunohistochemistry. This shielding effect w...

AI Technical Summary

Problems solved by technology

There are many types of immunohistochemical antibodies used in the clinical pathology department, and there are also many different suppliers at home and abroad, so the paired application of different antigen retrieval solutions and a wide variety of antibodies greatly increases the workload of the clinical pathology department, and if the wrong application If the antigen retrieval solution is used, it will also lead to the risk of detection failure

[0008] Due to the use of different kinds of antigen retrieval solutions for different antibodies, it is very inconvenient for clinical pathological immunohistochemical detection, and there is a considerable risk of detection failure due to considerable application errors

Among the many existing antigen retrieval solutions, they can only be applied to some antibodies

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